小麦温敏核雄性不育系BNS育性转换关键调控基因发掘

项目名称： 小麦温敏核雄性不育系BNS育性转换关键调控基因发掘

项目编号： No.31301002

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 生物科学

项目作者： 董娜

作者单位： 河南科技学院

项目金额： 22万元

中文摘要： 杂种优势利用是提高作物产量改善作物品质的重要途径。BNS是河南科技学院茹振钢教授培育的低温敏感型核雄性不育系，在广大适宜区域内表现"不育彻底、转换彻底、恢复彻底、育性转换稳定"等显著特征，其制种不育度和自繁可育度均达到生产上规模化杂交制种与繁种要求，是黄淮麦区二系杂交小麦的新突破，但其育性转换的分子机制尚缺乏系统研究。本项目的研究内容：以BNS幼穗为材料，利用转录组测序技术结合遗传群体分析，研究低温敏感时期BNS在可育与不育条件下的基因表达差异，发掘其育性转换的关键调控基因。本项目首次从分子水平上研究BNS在可育与不育条件下的基因表达差异，进而发掘其育性转换的关键调控基因，这将为阐明BNS育性转换的分子机制以及开发功能性的分子标记奠定基础。

中文关键词： 小麦；BNS型雄性不育系；育性转换；调控基因；转录组测序

英文摘要： Heterosis utilization is one of the most important methods to increase crop yield and improve its quality. BNS is a thermo-sensitive genic male sterile wheat line bred by Professor Ru zhen-gang from Henan Institute of Science and Technology. In the suitable growth areas, BNS could show absolute male sterile or complete normal fertility if the temperature was suitable at its thermo-sensitive stage, and that the fertility transition pattern was steady in different years. The male fertility of BNS can also be completely restored by some normal cultivars. Additionally, the male sterile degree and the male fertile one have met the requirements for large-scale producing hybrid and self-production. So, BNS may be a breakthrough in the research of two-line hybrid wheat in Huang-huai wheat growth area. But till now, the molecular mechanism of the fertility alteration of BNS has not yet been systematically studied. In this study, using RNA-seq method together with group analysis, we will try to discover genes crucial for regulating the fertility alteration of BNS through analysis the differences of gene expression of BNS in thermo-sensitive phages under sterile and fertile conditions, which will highlight the understanding of molecular mechanism of the fertility alteration of BNS and development of functional markers for

英文关键词： wheat；BNS-type male sterile line；fertility alteration；regulation gene；RNA-seq