低剂量及复合个人护理品暴露的分子防御干扰效应和早期预警研究

项目名称： 低剂量及复合个人护理品暴露的分子防御干扰效应和早期预警研究

项目编号： No.21277092

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 环境科学、安全科学

项目作者： 袁涛

作者单位： 上海交通大学

项目金额： 80万元

中文摘要： 识别新生污染物低剂量/环境相关浓度和复合暴露的生态毒性和健康效应是相关领域迫切需要解决的关键科学问题之一。本项目结合前期研究积累和实验数据，跟踪细胞生物学和分子生物学的最新动态以及大科学装置提供的前所未有的机遇，从生物体敏感的分子防御多耐药通路，研究个人护理品成分（有机紫外防晒剂和有机硅氧烷）低剂量以及复合污染暴露时，对模式生物和人体细胞分子防御功能、转运子蛋白以及相关基因水平的影响；并利用同步辐射技术，从微观角度检测细胞形态结构、行为生理、元素分布变化，探索目标物低剂量暴露效应的早期预警信号和分子机制。本项目将为个人护理品生态风险和健康风险评价提供重要科学数据；分子防御通路的研究也为揭示污染物复合暴露效应、低剂量毒物兴奋效应（Hormesis）和适应性反应（Adaptive response）的作用机制提供新的思路和切入点；而同步辐射技术的引入，有助于促进分子毒理学领域的源头创新和突破。

中文关键词： 个人护理品；嗜热四膜虫；基因表达；外源化合物耐受；分子对接

英文摘要： It is the critical key issue in the field of molecular toxicology to identify the adverse effects of low-dose (or environmental relevant concentration) and joint exposure of the emerging contaminants. The idea of project is based on the groups strong previous studies and research data, the cutting edge development in cell and molecular biology and the advantage of the mega science device. The project is to investigate the adverse ecological and health effects of mode organism and human cell exposed to low-dose (or environmental relevant concentration) and mixtures of personal care products, thru the sensitive molecular efflux transporter pathway, which including cell efflux function,relevant protein activity and gene expression, etc. On the other hand, synchron radiation technology will be introduced to study the morphology, physiological behaviors and element distributions of the selected organism and human cell line in the micro scale, to disclose the early warning signals and molecular mechanisms of low dose exposure. This project will provide the important scientific information for the ecological and health risk assessment of personal care products. By the systematic studies of the molecular efflux defense pathway, it indicates a new clue and entry point to understand the mechanisms of joint exposures effe

英文关键词： personal care products；Tetrahymena thermophila；gene expression；multixenobiotic resistance；molecular docking