绿豆芽萌发过程维生素C积累的代谢调控机制

项目名称： 绿豆芽萌发过程维生素C积累的代谢调控机制

项目编号： No.31501765

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 农业科学

项目作者： 郭新波

作者单位： 华南理工大学

项目金额： 20万元

中文摘要： 绿豆芽的生产周期短、营养价值高，是我国需求量最大的芽苗菜。研究表明，绿豆在萌芽过程中其维生素C的含量可急剧增高（24倍），维生素C积累明显增强绿豆芽抗氧化品质，但与之相关的机理，未见较系统和深入的研究。本人所在实验室通过对维生素C代谢调控研究发现，植物维生素C的L-半乳糖合成途径功能基因表达调控对维生素C合成有明显影响，增强抗坏血酸还原酶基因的表达能提高维生素C的再生循环。因此，推测萌芽可能通过激活绿豆芽维生素C的L-半乳糖合成途径和再生循环，导致绿豆芽维生素C含量显著提升。本项目拟通过转录组测序筛选绿豆芽维生素C代谢调控相关功能基因，分析功能基因的表达模式，构建绿豆芽维生素C代谢通路，探明影响绿豆芽维生素C增高的主要因素及其过程，为优化绿豆芽生产工艺和强化营养品质提供理论依据。

中文关键词： 绿豆芽；维生素C；转录组；萌芽；调控网络

英文摘要： Mung bean sprouts are the largest demand of vegetables in China because of short production cycle and high nutritional value. Our previously research showed that vitamin C content was dramatically increased in mung bean sprouts during germination (almost 24 folds), and the antioxidant quality of mung bean sprouts was significantly enhanced followed by the rising of vitamin C. However, the regulatory mechanism of vitamin C metabolism was not systemically and deeply studied in mung bean sprouts. The research experiences on vitamin C metabolic regulation in our lab showed that regulation of key-encoding genes in L-galactose pathway was significant effect on vitamin C biosynthesis, and over-expression of ascorbate reductase also improved the regeneration cycle of vitamin C in plant. Our hypothesis is that L-galactose biosynthetic pathway and regeneration cycle for vitamin C accumulation was activated by germination in mung bean sprouts. The proposal aims to study the regulatory mechanism of vitamin C biosynthesis and regeneration in mung bean sprouts during germination. Functional genes screening related to vitamin C metabolism in mung bean sprouts will be analyzed by RNA-seq technique. Gene expression patterns and enzyme activities related to vitamin C metabolism will be analyzed in mung bean sprouts. Upon the comprehensive evaluation of functional gene expression profiles and key enzyme activities in mung bean sprouts, the metabolic regulatory network of vitamin C will be revealed in our study. The expected results will provide theoretical guidance and technical support for production technology optimization and nutrition quality fortification for mung bean sprouts, and will have great social economic value for agricultural processing.

英文关键词： Mung bean sprouts;Vitamin C;Transcriptome;Germination;Regulatory network