基于可磁分离时间分辨荧光传感体系的持久性有机污染物检测技术及机理研究

项目名称： 基于可磁分离时间分辨荧光传感体系的持久性有机污染物检测技术及机理研究

项目编号： No.21507034

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 化学工业

项目作者： 黄大伟

作者单位： 生态环境部华南环境科学研究所

项目金额： 20万元

中文摘要： 持久性有机污染物（POPs）可对人体产生严重危害，是目前环境监测和治理的焦点，开展对痕量POPs的快速灵敏检测能为污染的有效防治提供有力保障。时间分辨荧光分析法（TRFA）灵敏度高、抗干扰能力强，具备发展成实时在线监测的潜力。本项目拟通过对TRFA进行磁性强化，构建可磁分离时间分辨荧光传感体系（MSTRFSS），实现对痕量POPs的快速灵敏检测与传感体系的循环使用。首先，通过系统考察磁性纳米材料和长寿命荧光材料的制备、表面功能化、以及两者的复合方法，得到具有最佳磁性能和荧光性能的磁性/荧光纳米材料；其次，筛选合适的时间分辨荧光传感体系，探究其与磁性/荧光纳米材料的结合方式以构建MSTRFSS，并对MSTRFSS在POPs检测中的效能与作用机理展开研究；最后，明确MSTRFSS再生方法和再生机制。本研究将有助于痕量POPs快速检测技术的进一步发展和完善，对水体水质监测和控制具有重要意义。

中文关键词： 磁性分离；时间分辨荧光；生物传感器；持久性有机污染物；效能与机理

英文摘要： Persistent organic pollutants (POPs) is harmful to people's health, to which the current environmental monitoring and controlling give a lot of attention. The rapid procedure for the detection of trace POPs can provide strong guarantee for effective environmental controlling. Time-resolved fluorescence assay (TRFA) not only has the advantages of high sensitivity and strong anti-interference capacity, but also has the potential for real time online monitoring. This project will combine the TRFA and magnetic nanomaterials to fabricate magnetically separable time-resolved fluorescence sensing system (MSTRFSS), and aim at realizing the trace detection with specificity and the recycling of the sensing system. Through the study of the method for the magnetic nanomaterials and long lifetime fluorescence materials preparation, surface functionalization, and their composition, this project will obtain magnetic/fluorescence nanomaterials with the best magnetic and fluorescence properties. In order to develop MSTRFSS, the project will screen out suitable time-resolved fluorescence sensing system firstly, and then study the combine technique with magnetic/fluorescence nanomaterials. The detection efficiency and mechanism of interaction for POPs will be studied subsequently. Finally, the regeneration method and mechanism will also be studied. The research of this project will help to the improvement of the trace POPs rapid detection technology and will be of great significance for monitoring and controlling water quality.

英文关键词： magnetic separation;time-resolved fluorescence;biosensor;persistent organic pollutants;efficiency and mechanism