非平衡等离子体对粪肠球菌生物膜QS-fsr系统的作用研究

项目名称： 非平衡等离子体对粪肠球菌生物膜QS-fsr系统的作用研究

项目编号： No.81500847

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 医药、卫生

项目作者： 杜田丰

作者单位： 郑州大学

项目金额： 18万元

中文摘要： 群体感应（QS）系统能控制细菌间的信息传导，在细菌生长过程中扮演了重要角色，对调节生物膜形成、发展及成熟起着至关重要的作用。粪肠球菌生物膜的群体感应系统主要是fsr系统。我们的前期实验结果提示非平衡等离子体对粪肠球菌生物膜具有良好的灭活作用，但其作用机制还有待进一步探讨。为此，我们提出假说：非平衡等离子体可能通过干预QS-fsr系统的调控作用，从而抑制粪肠球菌生物膜的生长。为了验证这一假说，我们将通过建立粪肠球菌生物膜和感染牙本质小管体外模型以及牙髓根尖周病动物模型，采用SEM、CLSM、流式细胞术、real-time PCR、western blot、免疫荧光等手段，明确非平衡等离子体抑制粪肠球菌生物膜的重要作用，初步探讨其对QS-fsr系统的作用机制。本研究将从干预QS-fsr系统这个新视点为揭示非平衡等离子体抑制细菌生物膜的作用机制奠定基础，为牙髓根尖周病的治疗提供新的思路。

中文关键词： 牙髓根尖周病；粪肠球菌；细菌生物膜；非平衡等离子体；群体感应

英文摘要： Quorum-sensing (QS) system controls the signal transduction among bacteria, and plays a key role in regulating bacterial biofilms formation, development and maturation during bacterial growing. The fsr quorum-sensing (QS-fsr) system is an important one in Enterococcus faecalis biofilms. In our previous studies, it has been revealed that nonequilibrium plasma has the antibacterial activity against Enterococcus faecalis biofilms effectively. However, the mechanism of nonequilibrium plasma killing biofilms cells is still unknown. We hypothesize that nonequilibrium plasma may destroy the regulation effect of QS-fsr system, and inhibit Enterococcus faecalis biofilms growing. To conform this hypothesis in this project, Enterococcus faecalis biofilms and infected dentinal tubules in vitro, and apical periodontitis in rats are established. The techniques of SEM, CLSM, flow cytometry, real-time PCR, western blot, and immunofluorescence are used to reveal the effect of nonequilibruim plasma killing Enterococcus faecalis biofilms cells, and indicate the mechanism of nonequilibrium plasma against QS-fsr system. This is a new viewpoint of preventing QS-fsr system in the present project. It is aimed to provide a wild theoretical basis for studying the mechanism of nonequilibrium plasma killing bacterial biofilms cells, and a novel choice in therapy of endodontic diseases.

英文关键词： endodontics diseases;Enterococcus faecalis;bacterial biofilms;nonequilibrium plasma;quorum-sensing