莲花期候选基因关联分析与功能标记开发

项目名称： 莲花期候选基因关联分析与功能标记开发

项目编号： No.31200268

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 微生物学、植物学

项目作者： 杨美

作者单位： 中国科学院武汉植物园

项目金额： 22万元

中文摘要： 莲作为重要的观赏水生植物，于酷夏绽放（6-8月），错过了春秋两季赏花好时节，未能充分体现其观赏价值。因此，充分发掘莲不同花期优异基因资源，对于培育早花（花期提前到5月）或晚花（花期延迟至10月）莲品种具有重要意义。本项目拟采用已构建的莲核心种质库为关联分析群体，利用AFLP、SSR和SRAP分子标记评价其群体结构；根据中国古代莲全基因组测序所得开花关键基因CO、FT的序列设计引物，分析群体的基因等位变异；开展关联分析，检测与莲花期关联的基因等位变异位点；分别根据对早花或晚花有益的变异位点开发特异的功能标记，并利用杂交后代分离群体对功能标记进行检测和验证。本研究通过分析开花基因的等位变异探讨莲在长期进化过程中的演化规律，揭示莲花期遗传变异机理，所得功能标记为早花或晚花莲品种的选育提供直接有效的分子选择依据。

中文关键词： 莲；花期；FT gene；关联分析；功能标记

英文摘要： Lotus, an important ornamental aquatic plant, blooms in summer (from June to August), which misses the optimum timing to enjoy flower of lotus in spring and autumn and fails to reflect its ornamental value. Exploration of exotic gene resources with different flowering time has improtant significance for breeding new lotus varieties with early (bloom start from May) or late (last to October) flowering time. Taking the core collection of lotus as the association population, the present project is to survey the structure of the association population using AFLP, SSR and SRAP markers; Design primers according to the sequences of the flowering key genes, CO and FT, which blast from the genome sequence of 'Chinese Antique Lotus', and examine allelic variations in the association population; Test associations of flowering time with allelic loci, and develop functional markers based on the loci which contribute to early or late flowering time; Verify the applicability of functional markers linked to flowering time in the F1 segregating progenies. The project will analyze allelic variations of CO and FT to explore the evolution mechanism of lotus in the long evolution process, and to reveal the genetic variation of flowering time. The obtained functional markers provide useful information for molecular breeding of lotus

英文关键词： lotus；flowering time；FT gene；Association analysis；Functional marker