叶绿素合成途径HEMB1基因调控植物免疫的机理研究

项目名称： 叶绿素合成途径HEMB1基因调控植物免疫的机理研究

项目编号： No.31300206

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 生物科学

项目作者： 唐为江

作者单位： 中国科学院植物研究所

项目金额： 24万元

中文摘要： 叶绿素合成与植物免疫是植物生长中两个重要的过程，人们对两者关系的认识知之甚少。我们过去的研究发现了FHY3/FAR1转录因子直接结合并调控叶绿素合成途径基因HEMB1的表达，揭示了光调控叶绿素合成的分子机理。我们进一步观察到fhy3far1双突变体植株矮小、叶片卷曲、活性氧和水杨酸积累，以及出现自发性类病斑，并且过量表达HEMB1能恢复fhy3far1突变体的上述表型，暗示FHY3/FAR1、HEMB1及叶绿素合成与植物免疫可能密切相关。本项目拟深入分析FHY3/FAR1和HEMB1与活性氧及自发性类病斑形成的关系；研究HEMB1、叶绿素合成途径调节水杨酸合成及其信号和免疫的分子机制；分析HEMB1蛋白定位，及其结构域与植物免疫的关系；以及通过研究HEMB1互作蛋白，建立HEMB1调控免疫的可能网络与作用机制，为揭示叶绿体功能与植物免疫的相互联系提供实验证据和理论基础。

中文关键词： 活性氧；四吡咯生物合成；植物免疫；FHY3/FAR1；EDS1

英文摘要： Chlorophyll biosynthesis and plant immune are two important processes for plant growth. Little is known about the relationship between them. Our previous study demonstrate that two transcription factors FHY3 and FAR1 directly bind to the promoter of HEMB1, encoding an important enzyme of chlorophyll synthetic pathway, and promote its expression, thus revealing the molecular mechanism of light regulation of chlorophyll biosynthesis. We further observed that the fhy3far1 mutant plants are semi-dwarf with curly leaves, spontaneous lesions and accumulation of reactive oxygen species (ROS) and salicylic acid (SA). Strikingly, over-expression of HEMB1 is able to rescue these defects of fhy3far1 mutant. These findings suggest that FHY3/FAR1, HEMB1 and chlorophyll synthesis might play roles in regulating plant immune response. In this study, we propose to further analyze the relationship between FHY3/FAR1 and HEMB1 with the production of ROS and spontaneous lesions, and to elucidate the molecular mechanism of HEMB1 and chlorophyll biosynthetic pathway in regulating SA biosynthesis and its signaling and plant immune. Furthermore, we will analyze the subcellular localization of HEMB1 protein, and the relationship between its functional domains with plant immune response. Moreover, we will study the interacting proteins of

英文关键词： ROS；Tetrapyrrole biosynthesis；Plant immunity；FHY3/FAR1；EDS1