中空核壳结构的稀土上转换发光介孔复合材料及其靶向光动力学治疗应用

项目名称： 中空核壳结构的稀土上转换发光介孔复合材料及其靶向光动力学治疗应用

项目编号： No.21201163

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 无机化学

项目作者： 卢珊

作者单位： 中国科学院福建物质结构研究所

项目金额： 25万元

中文摘要： 光动力学治疗(PDT)作为肿瘤治疗的一种新技术，目前在应用上受到组织深度的限制，并且大多数PDT药物(即光敏剂)生物相容性差、靶向性有限。本项目致力于研发中空核壳结构的稀土上转换发光介孔复合材料，使其同时具备荧光探针、肿瘤靶向和药物负载等功能。通过稀土纳米晶的二氧化硅多级包覆和选择性腐蚀构筑中空核壳结构，提高上转换发光效率和光敏剂负载量，并引入有机介孔壳层避免光敏剂的聚集和猝灭；同时利用一种新型的广谱肿瘤标志物尿激酶受体(uPAR)与尿激酶氨端片断(ATF)的特异性结合实现材料的肿瘤靶向功能；最终达到上转换肿瘤成像与PDT的多功能应用。此外，利用高分辨激光和荧光光谱等手段揭示该材料的上转换发光和能量传递效率与其核壳结构的内在关系。预期本项目研制的中空核壳上转换发光介孔复合材料在PDT治疗效率、特异性以及组织穿透深度都具有显著优势，对多功能纳米材料在肿瘤诊疗及成像一体化的应用研究有重要意义。

中文关键词： 上转换发光；中空核壳结构；有机二氧化硅；光动力学治疗；多模成像

英文摘要： As an emerging technology for tumor therapeutics, photodynamic therapy (PDT) is limited by the tissue depth as well as the poor biocompatibility and targeting for the most of PDT drugs (photosensitizers). Therefore，we design a novel mesostructured rattle-type lanthanide-doped upconversion luminescence material, with multifunctions of fluorescent probing, tumor targeting and drug loading. The novel rattle structure and the mesoporous organosilica shell are introduced to improve the luminescence of upconversion nanocrystals and the loading capacity of photosensitizers, and further to avoid the aggregation and quenching of the hydrophobic photosensitizers. By use of the high affinity of amino-terminal fragment (ATF) of urokinase plasminogen activator (uPA) and uPA receptor (uPAR), the material is tumor-targeting and can thus be applied in simultaneous imaging and efficient photodynamic therapy. The relationship between upconversion luminescent efficiency, energy transfer process of the material and the core-shell structure is also revealed. This rattle-type multifunctional material is expected to be advantageous in PDT efficiency,specificity, and availability in deep tissues. The work is also of great significance in developing multifunctional nanoparticles for tumor diagnosis, imaging and therapy.

英文关键词： Upconversion luminescence；rattle structures；organosilica；photodynamic therapy；multimodal imaging