荧光/磁性纳米球用于循环肿瘤细胞的高灵敏检测

项目名称： 荧光/磁性纳米球用于循环肿瘤细胞的高灵敏检测

项目编号： No.21505157

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 数理科学和化学

项目作者： 温聪颖

作者单位： 中国石油大学（华东）

项目金额： 21万元

中文摘要： 循环肿瘤细胞（CTCs）的检测对肿瘤的早期诊断、治疗过程监测、预后、转移复发的早期监测等具有重要的意义。由于CTCs存在于复杂的外周血中，且浓度极低，所以CTCs的分离和富集是检测的关键步骤。本项目拟通过在荧光/磁性纳米球表面修饰多种能特异性识别CTCs的靶向分子，构建针对CTCs多种靶标的荧光/磁性纳米探针，进而基于该探针建立快速、灵敏、可靠检测肿瘤细胞的方法。在该法中，一方面，多种靶向分子的联用相比于使用单一靶向分子，能更充分地利用细胞表面的多种抗原，从而改善由于肿瘤细胞异质性或表面抗原丢失造成的“漏捕”问题，提高肿瘤细胞的捕获率；另一方面，利用纳米球的磁性功能和荧光功能同时完成CTCs的捕获和鉴定，相比于目前常用的先捕获后鉴定的方法，简化了操作步骤，节省了人力和时间，同时洗涤次数的减少降低了CTCs的损失率。因此，本项目能够在很大程度上促进CTCs的灵敏可靠检测，具有重要的医学意义。

中文关键词： 荧光/磁性纳米球；捕获；检测；循环肿瘤细胞

英文摘要： The detection and investigation of circulating tumor cells (CTCs) are of great significance to early diagnosis of tumors, treatment monitoring, prognosis, and metastasis diagnosis, in which isolation and enrichment of CTCs are key steps due to their extremely low concentration in peripheral blood. In this proposal, fluorescent/magnetic nanospheres will be modified with multiple kinds of targeting molecules which can specifically recognize CTCs to fabricate multi-targeting fluorescent/magnetic nanoprobes. The obtained nanoprobes will be then applied to the rapid, sensitive, and reliable detection of tumor cells. Compared with using a single kind of targeting molecule, combination of multiple molecules will be able to associate with the multiple antigens on the cells, which will enhance the binding between the tumor cells and the bioprobes. This will ameliorate the “CTC miss” problem arising from the heterogeneity of tumor cell antigen expression and evolving changes, which will improve the capture efficiency of tumor cells. On the other hand, fluorescent/magnetic nanospheres will facilitate the one-step detection of CTCs, in which the magnetic capture and the fluorescence identification can be accomplished simultaneously. Compared with the two-step detection strategy, which includes capture and a subsequent identification process, the one-step strategy will simplify the detection process, save labor and time, and reduce the CTC loss caused by washing steps. Thus, this research will greatly benefit the sensitive and reliable CTC detection, which is of important medical significance.

英文关键词： fluorescent/magnetic nanospheres;capture;detection;circulating tumor cells