基于凋亡蛋白合成元蘑多糖对辐射诱导脾细胞凋亡防护机制研究

项目名称： 基于凋亡蛋白合成元蘑多糖对辐射诱导脾细胞凋亡防护机制研究

项目编号： No.31500652

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 生物科学

项目作者： 李小雨

作者单位： 燕山大学

项目金额： 20万元

中文摘要： 辐射损伤现已渗透到人们生活的各个领域，寻找出高效、无毒、适宜人体长期服用的天然辐射防护剂仍是一个亟待解决的科研难题。研究发现多种天然产物能够对辐射诱导的机体损伤发挥防护作用，然而这些研究仅从对凋亡蛋白表达的影响角度揭示辐射防护机制，而对于其基于内质网途径中凋亡蛋白合成的防护作用机制还鲜有报道。前期研究发现水溶性元蘑多糖（NTHSP）具有显著的辐射防护功效，能够有效的抑制辐射诱导脾细胞周期阻滞和脾细胞凋亡，然而其防护机制仍不清楚。因此，本项目基于崭新的凋亡蛋白合成理论深入研究NTHSP对辐射诱导脾细胞内质网凋亡途径的防护作用，全面评价辐射对小鼠脾细胞中相关凋亡蛋白表达的影响，并对脾细胞中凋亡蛋白mRNA的表达图谱及相关核糖体进行分析，探索其在应激过程中迁移和表达的动力学规律，从凋亡蛋白翻译合成的角度揭示NTHSP对辐射损伤的防护作用机制，为NTHSP在医疗保健领域中的应用提供理论和实验依据。

中文关键词： 元蘑多糖；蛋白合成；细胞凋亡；防护机制；辐射

英文摘要： Radiation damage has penetrated into all fields of people's lives, so looking for the natural radioprotective agent with high efficient, non-toxic and suitable for human long-term use is still an urgent scientific problem. The literatures reported that a variety of natural products could protect the body against the damage induced by radiation. However, these researches only revealed the radioprotective mechanism in view of apoptosis protein expression, and the protective mechanism based on apoptosis protein synthesis in endoplasmic reticulum pathway is rarely reported. Previous study found that the water-soluble polysaccharides from Hohenbuehelia serotina possessed significant radioprotective effect, and could effectively inhibit the splenocyte cycle arrest and apoptosis. However, the protective mechanism is not clear. Therefore, with the new angle of apoptosis protein synthesis, this subject deeply investigates the protective activity of NTHSP on splenocyte apoptosis induced by radiation based on endoplasmic reticulum apoptosis pathway, comprehensively evaluates the effect of radiation on the related apoptosis protein expression profile of the mice splenocytes, analyzes the expression profile of apoptosis protein mRNA and the related ribosomes in splenocytes, and then explores the migration and expression kinetics of mRNA and ribosomes in stress process, thereby reveals the radioprotective mechanism of NTHSP on radiation damage from the angle of apoptosis protein translation, and provides theoretical and experimental evidence for the application of NTHSP in the health care field.

英文关键词： Hohenbuehelia serotina polysaccharide;Potein synthesis;Apoptosis;Protective mechanism;Radiation