胡萝卜光周期调控抽薹开花相关基因的发掘及功能分析

项目名称： 胡萝卜光周期调控抽薹开花相关基因的发掘及功能分析

项目编号： No.31272162

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 农业科学

项目作者： 庄飞云

作者单位： 中国农业科学院蔬菜花卉研究所

项目金额： 75万元

中文摘要： 胡萝卜是人类维生素A的重要来源。先期抽薹已成为我国冬春大棚和早春露地栽培可持续发展的瓶颈。由于缺乏适宜的研究材料，胡萝卜抽薹开花分子机制研究进展缓慢。项目组前期工作表明胡萝卜先期抽薹是受到短暂低温作用后，在长日照条件下诱导发生的。本项目以低温和长日照极为敏感的'松滋野生'、耐抽薹的栽培种'Amsterdam'及其构建的具有不同先期抽薹开花特性的回交重组自交系（BIL）为素材，建立胡萝卜光周期开花调控模型，采用RNA-seq技术分析不同光照长度处理后各试材转录组差异基因表达，富集分析胡萝卜抽薹开花途径的相关基因，通过在BIL系开花调控过程中的功能分析确定目标基因。本项研究为胡萝卜先期抽薹的防控、种质资源抽薹特性精准鉴定、耐抽薹品种选育提供一个崭新的理论和技术平台。

中文关键词： 胡萝卜；开花时间；光周期；低温；转录组测序

英文摘要： Carrot (Daucus carota L.) is an out-crossing biennial species, and one of the most economical and important vegetable crops in the world. The premature bolting resulting in a complete loss of carrot commercial value has been the key effect on hindering the development of winter-spring plastic tunnel cultivation and spring cultivation in China. Limited progress has been reported on the control of bolting and flowering in carrot due to lacking the suitable materials. Vernalization is a checkpoint which carrot must pass, where premature bolting could be induced and advanced by long days (LD) in our previous study. This control was similar with those of Arabidopsis and Beet. In addition, high-throughput transcriptomic sequencing is essential to generate a large transcriptome sequence dataset for gene discovery especially to carrot, a species lacking genomic resources. In the study, a photoperiod control of bolting and flowering would be established with the wild species 'Songzi' which is very sensitive to cold temperature and LD, 'Amsterdam' resistant to premature bolting, and their backcrossing inbreed lines (BIL) with different charactersitcs of premature bolting and flowering. The transcriptome of 'Songzi' and 'Amsterdam' dealed with different days of LD will be sequenced and assembled into unigenes. The unigenes

英文关键词： Carrot；Flowering time；Photoperiod；Low temperature；RNA-seq