SOA1在AtRBOHF-ROS抗盐途径和凯氏带发育调控途径中的功能分析

项目名称： SOA1在AtRBOHF-ROS抗盐途径和凯氏带发育调控途径中的功能分析

项目编号： No.31470350

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 生物科学

项目作者： 蒋才富

作者单位： 中国农业大学

项目金额： 85万元

中文摘要： 有研究表明凯氏带质外体屏障与Na+吸收调控和抗盐应答有关，但对其机制还了解甚少。我们之前的研究表明AtRBOH-ROS通过抑制Na+吸收来促进植物抗盐，AtRBOHF功能缺失突变体atrbohf-F3对高盐胁迫敏感。最近Lee等的研究表明AtRBOHF-ROS参与了凯氏带发育调控，AtRBOHF功能缺失突变体凯氏带发育存在缺陷。在此基础上，我们发现AtRBOHF-ROS介导抗盐与其调控凯氏带发育相关，并且筛选到了一个atrbohf-F3的隐性抑制突变体soa1：它既能抑制atrbohf-F3的高盐敏感表型又能抑制其凯氏带缺陷,推测SOA1是AtRBOHF途径的负调控基因。本项目拟在克隆SOA1的基础上，深入研究它在凯氏带发育调控和抗盐应答中的作用，相关研究结果将增进我们对AtRBOHF-ROS抗盐途径、凯氏带发育调控机制及其在高盐胁迫应答中的作用的认识,并对抗盐作物的培育有一定的指导意义。

中文关键词： AtRBOHF；凯氏带；抗盐；活性氧

英文摘要： Casparian strip forms a tight apoplatic barrier in the root endodermis of plant. Previous studies have indicated that the casparian strip barrier likely plays important roles in plant salinity tolerance, however, the mechanism is poorly understood. Our previous observations showed that reactive oxygen species (ROS) produced by AtRBOHF confers soil-salinity tolerance, the mutants lacking AtRBOHF function (atrbohf-F3) display a salinity hypersensitivity. Lee et al. found that AtRBOHF-ROS is also a key player regulating casparian strip development, the mutants lacking AtRBOHF function display a defective casparian strip. In additon, our pilot situdies indicated that AtRBOHF-ROS confers salinity tolerance partially via regulating casparian strip development. Building upon these discoveries, we characterized a novel suppressor mutant (soa1) that can supresses both the salinity hypersensitivity and casparian strip defect of atrbohf-F3. I now seek to discover the funtion of SOA1. The results of this study will substantially advance our understanding of the mechanisms by which AtRBOHF-ROS regulates casparian strip development and thus salinity tolerance, and may enable future advances in crop plant tolerance of soil salinity.

英文关键词： AtRBOHF;casparian strip;salinity tolerance;ROS