血管形成在颞下颌关节骨关节炎中的作用机制

项目名称： 血管形成在颞下颌关节骨关节炎中的作用机制

项目编号： No.81200804

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 医学二处

项目作者： 房维

作者单位： 武汉大学

项目金额： 23万元

中文摘要： 颞下颌关节骨关节炎（Osteoarthritis，OA）严重影响患者的生活质量。本课题根据OA关节退行性改变中血管形成这一病理特点，探讨血管形成在颞下颌关节OA中的作用机制。通过切除部分颞下颌关节盘建立颞下颌关节OA动物模型，明确颞下颌关节OA中血管形成时间和空间分布特点，包括血管形成相关因子（VEGF、MMPs、ChM-1等）的分布及表达特点，血管密度和分布等。进而通过慢病毒载体建立不同ChM-1表达水平的髁突软骨细胞，与髁突软骨下骨成骨细胞直接共培养，分析体外炎性环境中，不同ChM-1表达水平对髁突软骨血管形成相关因子和SOX-9、I、II、X型胶原表达的影响。最后通过颞下颌关节腔注射ChM-1慢病毒颗粒和ChM-1shRNA慢病毒颗粒，分析不同ChM-1表达水平对颞下颌关节OA血管形成和OA病变过程的影响。

中文关键词： 颞下颌关节；高迁移率族蛋白1；骨关节炎；软骨调节素1；血管内皮细胞生长因子

英文摘要： The osteoarthritis (OA) of temporomandibular joint(TMJ) leading to joint dysfunction reduces quality of life while the diseae mechanism is unknown.Degenerative changes including articular cartilage loss,synovial inflammation, fibrosis, subchondral bone remodelling and osteophyte formation can be found in OA. Angiogenesis, may contribute to each of these features.The purpose of this project will clarify the role of angiogenesis in TMJ OA.In this project,OA animal moadel will be built by patial discectomy . Analysis of angiogenesis, in cluding blood vessel formation,blood vessel density and expression of pro-angiogenic and anti-angiogenic factors, in osteoarthritic TMJ will be performed by HE staining,immunostaining ,qRT-PCR and western-blot.After that,the expression of pro-angigenic and anti-angiogenic factors will be detected when TMJ condylar chondrocytes are treated with IL-1β.And then lentivirus vectors are used to regulate the expression of chondromodulin-1(ChM-1),an anti-angiogenic factor,in TMJ chondrocytes.The chondrocytes will be co-cultured with subchondral osteoblasts under IL-1β and the experssion of pro-angigenic and anti-angiogenic factors ,matrix formation,sox-9，collagen I ,collagen II，collagen X et,al. will be detected.At last,the ChM-1 lentivirus particles and ChM-1shRNA lentivirus p

英文关键词： temporomandibular joint；high mobility group box-1；osteoarthritis；chondromodulin-1；vascular endothelial growth factor