癌变生物标志物与溃疡性结肠炎脾胃气虚证的相关性研究

项目名称： 癌变生物标志物与溃疡性结肠炎脾胃气虚证的相关性研究

项目编号： No.81202804

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 医学八处

项目作者： 汪芸

作者单位： 中国中医科学院广安门医院

项目金额： 23万元

中文摘要： 溃疡性结肠炎作为难治性疾病，发病率逐年增高。溃疡性结肠炎相关性大肠癌是该病的并发症之一，且预后不良。加强癌变危险因素的监测可以提高CAC的早期诊断率。中医认为溃疡性结肠炎病机表现为本虚标实的复杂证候，但总是以脾胃虚弱为主要的发病基础。结合已有研究，我们推测溃疡性结肠炎脾胃气虚证患者较其它证型患者具有更高的癌变风险。本课题拟从溃疡性结肠炎癌变生物标志物的变化入手，在器官-组织-细胞三个层次上观察患者结肠黏膜的病理形态，在基因及蛋白质水平分别测定结肠黏膜组织中p53、K-ras、APC的表达，并且检定黏膜组织内活性氧的含量，研究PI3K/Akt信号通路的活化情况。通过以上实验结果阐释脾胃气虚证所包涵的病理生理意义，为该证型的判断提供一定的科学依据。同时，这项研究对于参考中医证型评估患者预后，辅助监测病情发展也具有重要意义。

中文关键词： 溃疡性结肠炎；溃疡性结肠炎相关性大肠癌；脾胃气虚证；微小RNA；DNA甲基化

英文摘要： Ulcerative Colitis (UC) is a refractory disease, its incidence increased year by year. Ulcerative Colitis Associated Colorectal Carcinoma (CAC) is one of the complications of UC with poor prognosis. Regular surveillance of risk factors can improve the early diagnosis of CAC. Chinese medicine believes that the pathogenesis feature of UC is deficiency in origin and excess in appearance. However, deficiency of spleen qi and stomach qi is always the main pathological basis of UC. On the basis of existing research, we hypothesized that the patients with deficiency of spleen qi and stomach qi have a higher risk of developing CAC than the patients with other TCM syndromes. The project intend to start off from the CAC-related biomarkers, and then observe the morphology of the colonic mucosa, determination the gene expression and protein levels of p53, K-ras and APC in the colonic mucosa, measure the content of reactive oxygen species in the mucosa and study the activation of the PI3K/Akt signaling pathway on the levels of organs-tissue-cell. To explain the pathophysiological implications of deficiency of spleen qi and stomach qi and provide a scientific basis for the diagnosis of TCM syndromes. Meanwhile, this has important significance to assess the prognosis of patients and monitoring disease progression with TCM synd

英文关键词： Ulcerative Colitis；Ulcerative Colitis Associated Colorectal Carcinoma；spleen qi and stomach qi deficiency syndrome；microRNA；DNA methylation