基于跨键能量传递机理构建一氧化氮双光子比率荧光探针及其生物成像研究

项目名称： 基于跨键能量传递机理构建一氧化氮双光子比率荧光探针及其生物成像研究

项目编号： No.21502067

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 数理科学和化学

项目作者： 任明光

作者单位： 济南大学

项目金额： 21万元

中文摘要： 一氧化氮 (NO) 作为最简单的生物信息分子，在生物体内参与重要的生理和病理过程。要阐明NO分子的生理功能和病理机制，需要建立一个在活细胞和生物组织内对NO可视化的检测方法。NO双光子荧光探针能够在组织深层面进行限域激发，从而实现对NO在生物组织的三维成像。然而现有的NO双光子荧光探针的信号响应都是荧光增强型，信号分辨率低且容易受到外界因素的干扰。比率型NO双光子荧光探针可以解决上述问题。通过对本课题组构建的具有良好信号分辨率的单光子荧光平台进行创新，连接上具有优良双光子性能的双光子能量供体，构建具有良好信号分辨率的双光子荧光平台，并在此平台的基础上构建NO荧光探针。本项目旨在构建具有良好信号分辨率的比率型NO双光子荧光探针，测试其对NO识别的光谱性质，研究其在活细胞、生物组织内对NO的双光子三维成像中的应用。有望解决目前NO双光荧光探针信号分辨率低的问题，具有重要的科学意义和研究价值。

中文关键词： 荧光探针；比率型；一氧化氮；双光子；生物成像

英文摘要： Nitric oxide (NO) as the simple biological information molecular, participate important physiological and pathological processes in vivo. To elucidate the physiological functions and pathological mechanisms of NO molecules, need to create a visualization of NO detection in living cells and biological tissues. Two-photon fluorescence probe capable in the organization deep levels to spatially confined two-photon excitation, enabling three-dimensional imaging of NO in the living cells and tissues. However, the signal response NO existing two-photon fluorescence probes are the type of fluorescence turn-on, signal resolution is low and vulnerable to external disturbances. Ratiometric two-photon fluorescence probe can solve the problem. Through innovation the single-photon fluorescence platform which was our group built with a good signal resolution, to build new two-photon fluorescence platform with good resolution, and construct the fluorescent probes on the basis of platform. Aims to build a good signal resolution ratiometric NO two-photon fluorescence probes, testing their spectroscopic properties of NO identification and study its application in living cells and living tissue NO two-photon three-dimensional imaging, which has a good theoretical guidance and practical significance for developing two-photon ratiometric fluorescent probes with well-resolved emission spectra.

英文关键词： Fluorescent probe;Ratiometric;Nitric oxide;two-photon;Biological Imaging