基于宽带受激辐射的纳米光学成像技术研究

项目名称： 基于宽带受激辐射的纳米光学成像技术研究

项目编号： No.61335003

项目类型： 重点项目

立项/批准年度： 2014

项目学科： 无线电电子学、电信技术

项目作者： 丁志华

作者单位： 浙江大学

项目金额： 260万元

中文摘要： 超衍射极限的纳米光学成像技术，是生物医学光学成像技术的重要进展，必将推动生物医学研究的重大突破。然而，现有纳米光学成像技术在性能上仍存在不足，如成像速度尚需提高、不能针对非荧光样品等。本项目提出基于宽带光受激辐射的纳米超分辨光学成像机制，引入基于谱域光学相干层析技术的高灵敏度并行探测方法，以期实现对非荧光色团和常规荧光样品的亚百纳米分辨的快速成像。本项目在理论分析的基础上，设计并搭建纳米分辨光学成像系统，测试该新型纳米光学成像技术在实际中的成像效果，并开展相关的生物医学应用研究。本项目的实施将首次实现对于非荧光样品的超分辨显微成像，为非荧光标记纳米光学显微术的发展奠定基础，扩大纳米光学成像技术在生物医学研究中的应用，同时也为显著提高纳米光学成像技术的成像速度提供一种全新的思路。

中文关键词： 纳米显微术;受激辐射;光学相干层析术;;

英文摘要： Optical nanoscopy with a resolution beyond the conventional diffraction barrier, a big advance in biomedical optical imaging technologies, will certainly give rise to vital breakthrough in biomedical researches. However, the performance of existing nanoscopy is still need to be improved, including its imaging speed, and feasibility for non-fluorescent samples. The project introduces stimulated emission of wideband light for optical superresolution, using spectral domain optical coherence tomography for parallel high-sensitive signal detection. The proposed novel optical nanoscopy can achieve fast optical imaging at a resolution of sub-hundred nanometers for both non-fluorescent chromophores and fluorescent samples. Based on theoretical analysis, we design and build the proposed system, test the performance of the constructed system and conduct several related biomedical researches. The implementation of the project not only realizes nanoscopy of non-fluorescent samples for the first time, laying foundation for further development of label-free optical nanoscopy and extending its application in biomedical researches, but also provides a novel concept for enhancement of imaging speed for optical nanoscopy.

英文关键词： nanoscopy;stimulated emission;optical coherence tomography