ABA调控冬小麦糖代谢应答低温胁迫的生理分子机制研究

项目名称： ABA调控冬小麦糖代谢应答低温胁迫的生理分子机制研究

项目编号： No.31471423

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 食品科学、农学基础与作物学

项目作者： 苍晶

作者单位： 东北农业大学

项目金额： 75万元

中文摘要： 东农冬麦1号(dn1) 是我国首例能在黑龙江省高寒地区越冬的强抗寒(可耐-30℃低温)冬小麦 (返青率＞85%),是耐寒研究珍贵材料。植物的糖代谢参与低温胁迫应答。我们前期研究发现,低温可促进冬小麦糖含量及代谢相关酶活性增加,且这些变化受外源ABA影响可增强抗寒性,但作用机制尚不清楚。本项目以dn1为材料,ABA处理后,大田自然降温条件下取样分蘖节,采用生理生化及分子生物学手段,检测糖含量及代谢关键酶活性与酶基因表达情况,明确ABA调控冬小麦糖代谢的生理机制；在此基础上,克隆关键酶基因,验证功能；同时通过双向电泳和质谱技术获得与ABA调控相关的差异蛋白,生物信息学分析,确定其功能和参与的代谢途径、信号途径及蛋白互作网络。综合糖代谢生理机制、基因分析和蛋白组学预测,揭示ABA调控冬小麦糖代谢应答低温胁迫的生理分子机制。项目有高寒地区冬麦研究特色,结果可为抗寒分子育种及栽培调控提供新思路及基因

中文关键词： 冬小麦；外源ABA；糖代谢；低温胁迫；应答机制

英文摘要： Dongnongdongmai 1(dn 1) was the first freezing-tolerant(resistant to low temperature of -30℃)winter wheat that can live through the winter in extreme frigid region in Heilongjiang Province.The reviving rate was greater than 85% which was considered to be a precious material for cold research. Sugar metabolism in plant took part in the response to cold stress. Our previous study indicated that low temperature could increase the sugar content and the activity of related metabolism enzymes in winter wheat. In addition, all the changes were affected by exogenous ABA, but the mechanism was not cleared.dn1 was used as materials, after dealt with ABA, the tillering nodes were sampled under natural cooling condition in fields. Sugar content and the expression pattern of enzyme genes will be tested using the physiological, biochemical and molecular levels methods., and then the physiological mechanisms of ABA regulating sugar metabolism in winter wheat were cleared. Based on these, the key enzyme genes will be cloned, and their functions be verified. Two-dimensional electrophoresis will be used to acquire differential proteins which are related to ABA regulation. After bioinformatic analysis, the functions, metabolic pathways, signaling pathways and protein - protein interaction networks involved will be determined. The physiological and molecular mechanisms of ABA regulation of sugar metabolism in winter wheat in the response of low temperature stress are revealed by integrating the physiological mechanisms, gene analysis and proteomics forecast. Our project is featured by researching on winter wheat in extreme frigid region. The results could supply new ideas for cold-resistance molecular breeding and cultivation, and rich cold-resistance genetic resources.

英文关键词： winter wheat;exogenous ABA;sugar metabolism;low teperature stress;Response mechanism