W06A7.4基因在MDHB延缓线虫衰老中的作用及其机制研究

项目名称： W06A7.4基因在MDHB延缓线虫衰老中的作用及其机制研究

项目编号： No.81473296

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 医药、卫生

项目作者： 罗焕敏

作者单位： 暨南大学

项目金额： 86万元

中文摘要： 本课题组前期研究证实，3,4-二羟基苯甲酸甲酯(MDHB)可明显延缓秀丽隐杆线虫衰老，同时伴有W06A7.4基因(与人类跨膜蛋白144基因同源)的高表达。本研究拟探讨W06A7.4基因是否为MDHB的靶基因？MDHB延缓线虫衰老是否通过W06A7.4基因的高表达来实现？目前，线虫W06A7.4基因的功能未知。本研究将采用基因组学的方法，包括基因敲除/敲入(CRISPR/Cas9法)、基因沉默(RNAi)、基因转染、定点突变、qPCR等，以探明MDHB是否通过改变W06A7.4基因表达发挥延缓线虫衰老的作用；W06A7.4基因是否为一潜在的长寿基因。本项目将在基因水平阐明MDHB延缓线虫衰老的机制，它将有助于MDHB进一步开发利用。同时，本项目还将着重阐明W06A7.4基因的重要功能意义，由于该基因与人类有同源基因，其重要功能的发现还将有助于对人类某些衰老性疾病如神经退行性疾病的认识和防治。

中文关键词： W06A7.4基因；3；4-二羟基苯甲酸甲酯；秀丽隐杆线虫；抗衰老；机制研究

英文摘要： Preliminary study of our research group confirmed that Methyl 3,4-dihydroxybenzoate (MDHB) obviously retarded aging in C.elegans with high expression of W06A7.4 gene ,the Homo sapiens orthologs of which is transmembrane protein 144 gene. At present, the function of W06A7.4 in C.elegans remains unknown. This study is to investigate whether W06A7.4 is a target gene of MDHB and whether MDHB displays antiaging effects through the overexpression of W06A7.4 gene. In this study, genomics methods, including CRISPR/Cas9, RNAi, gene transfection, site-directed mutagenesis and qPCR will be used to investigate whether MDHB retards aging in C.elegans by alterations in the expression of W06A7.4 gene and whether W06A7.4 is a potential longevity gene. This study is to elucidate the mechanism of the antiaging effects of MDHB in C.elegans at gene level, so as to provide reference basis for further development and utilization of MDHB. Since W06A7.4 is homological to Homo sapiens transmembrane protein 144 gene, it is of great significance to study the function of W06A7.4 gene，which will be conducive to the recognition and prevention of age-related disease, such as neurodegeneration disease.

英文关键词： W06A7.4 gene;methyl 3;4-dihydroxybenzoate(MDHB);caenorhabditis elegans;antiaging;mechanism research