项目名称: 基于聚集诱导发光的喹啉腈类功能染料及其荧光传感、活体影像研究
项目编号: No.21476076
项目类型: 面上项目
立项/批准年度: 2015
项目学科: 有机化学
项目作者: 朱世琴
作者单位: 华东理工大学
项目金额: 83万元
中文摘要: 聚集荧光淬灭效应已成为制约有机荧光功能染料在生物体活细胞和组织中应用的瓶颈之一。本项目利用喹啉腈类化合物独特的聚集诱导发光性能(AIE),围绕荧光分子-主客体-微环境检测,充分发挥化学荧光探针信号的传导优势,重点发展长波长、近红外AIE 活性的喹啉腈类发色团,拓展长波长AIE 体系,克服现有AIE体系的不足;依据活性物种小分子的自身特点,针对性地设计具有肿瘤靶向性的荧光聚集增强型近红外传感染料,通过荧光单元和识别基团的优化组合构建AIE 活性分子型荧光传感检测体系;期望解决分子型荧光传感染料的扩散性、自聚集荧光淬灭、光漂白现象等制约其在生物成像中实际应用的问题,建立和发展具有鲜明医学诊断特色的、可应用于细胞内重要客体分子、细胞微环境变化检测与影像的光学探针设计方法,为前药缓释、荧光传感和活体影像的基础研究提供高性能功能染料。
中文关键词: 荧光探针;设计合成;聚集态荧光增强;喹啉腈;活体成像
英文摘要: The aggregation-caused quenching effect (ACQ) has become a thorny obstacle to design high performance organic fluorescent dyes. In this project, through delicate modification by molecular engineering way, a series of solid-state enhanced far red to near infrared emission dyes, that is aggregation-induced emission (AIE), based on quinolone-malononitrile have been designed. In particular, near-infrared active AIE fluorescent probes are built with focus of the sensing mechanism and the unique characteristics of small active targeted molecules. Furthermore, polymeric micelles are applied for immobilizing and loading small fluorescent dyes to form organic nanoparticles, which can overcome the restriction or limitation of small fluorescent probes applied in bioimaging, such as diffusion, self-aggregation and photo-bleaching phenomena. This proposal will provide a series of active AIE quinolone-malononitrile based functional dyes for prodrug bioimaging with high photostability and efficacy,and reduced side-effects,especially for in situ and in vivo tracking of antitumor chemotherapy in living animal models.
英文关键词: Fluorescence sensor;Synthesis;Aggregation-induced emission;Quinolinemalononitrile;Bioimaging