茶树不同外植体再分化潜力差异的表观遗传机制研究

项目名称： 茶树不同外植体再分化潜力差异的表观遗传机制研究

项目编号： No.31470687

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 农业科学

项目作者： 陆建良

作者单位： 浙江大学

项目金额： 84万元

中文摘要： 转基因技术是茶树基因功能鉴定、种质改良的重要手段，离体组织再分化困难是茶树转基因的主要技术瓶颈。但茶树离体组织再分化困难的内在分子机制目前仍不明确。本项目采用表观遗传学分析手段，比较不同再分化潜力茶树外植体及其脱分化和再分化衍生材料的基因组DNA甲基化、组蛋白修饰、sRNA组成及基因表达等表观遗传因子差异，分析DNA甲基化和组蛋白修饰抑制剂处理对外植体再分化潜力及表观遗传因子的影响，明确茶树外植体在脱分化和再分化过程中表观遗传因子的变化规律，探明不同外植体再分化潜力与DNA甲基化、组蛋白修饰等表观遗传因子之间的关联关系。为阐明茶树外植体脱分化和再分化分子调控机制创造条件，同时也可为建立和完善高效茶树再生和转化体系提供重要理论依据。

中文关键词： 茶树外植体；再分化潜力；表观遗传机制；DNA甲基化；组蛋白修饰

英文摘要： Transgenic technology is an important approach for identifying the function of new gene and improving germplasm of the tea plant. Redifferentiation frequency of the tissues is quite low in vitro culture of tea plant, which is the main bottleneck of application of transgenic technology. However, the molecule mechanism on the redifferetiation obstacle of tissues from the tea plant is still unclear. In this study, the epigenetic differences of the genomic DNA methylations, histone modifications, sRNA components and gene expression patterns will be extensively compared in the dedifferentiated and redifferentiated materials as well as in the initial tea explants with different regeneration potentials. The effects of the DNA methylation and histone modification inhibitors on the change of epigenetic factors and the redifferentiation potentials of the explants will also be tested thoroughly. The variation patterns of the epigenetic factors in the explants during the dedifferentiation and redifferentiation process will be uncovered.The relationship between redifferentiation potential of the different explants and their epigenetic factors will be established and elucidated finally. These findings will help to reveal the regulatory mechanisms in molecule level during the dedifferentiation and redifferentiation process of tea explants, and will also provide a theoretical basis for improving the regeneration and transformation system of tea plant.

英文关键词： Tea explant;redifferentiation potential;epigenetic mechanism;DNA methylation;histone modification