3个小麦种子特异表达的全新microRNA的功能研究

项目名称： 3个小麦种子特异表达的全新microRNA的功能研究

项目编号： No.31471482

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 食品科学、农学基础与作物学

项目作者： 赵惠贤

作者单位： 西北农林科技大学

项目金额： 85万元

中文摘要： 籽粒生长发育是小麦产量和品质的重要基础，揭示其分子调控机制可为小麦优质高产育种提供重要理论依据。miRNA是近年来发现的一类调控因子，调节内源基因表达，广泛参与植物生长发育，在基因调控网络中起关键调控作用。最近,我们在小偃6号小麦中鉴定出籽粒特异表达miRNA，它们如何调控籽粒的生长发育及品质性状形成尚不清楚。在前期研究基础上，本项目重点研究3个小麦籽粒特异表达新miRNA(tae-miR2026, tae-miR39和tae-miR15)的功能。拟对幼苗、旗叶和不同发育时期籽粒的RNA降解组进行高通量测序，确定候选miRNA的靶基因；分离克隆miRNA及其靶基因序列；利用病毒诱导小麦籽粒内源基因沉默和转基因过表达2种策略进行候选miRNA及其靶基因的功能鉴定。旨在揭示候选miRNA对籽粒生长发育的调控作用及调控方式，并希望能鉴定出在小麦优质高产育种上具有应用价值的miRNA或靶标基因。

中文关键词： 小麦；种子；microRNA；功能；调控网络

英文摘要： Wheat seed development is the most important base of its yield and quality, uncovering the regulatory molecular mechanism of wheat seed growth and development will provide scientific theory basis for wheat quality and high yield breeding. MicroRNAs (miRNAs) are a class of small RNAs that are the products of endogenous non-protein-coding genes and serve as sequence-specific trans-acting regulators for controlling eukaryotic gene expression at the post-transcription levels. They have been found to regulate a various biological process, such as plant growth, development, differential, and stress responses, and play key roles in gene expression and regulatory network. Recently, we identified some seed-specific novel miRNAs, however, the biological functions of these miRNAs are unknown. In this research project, we will focus on functional identification of three wheat seed-specific novel miRNAs, tae-miR2026, tae-miR39 and tae-miR15, based on our previous study. RNA degradoms from wheat seedlings, flag leaves, and developing seeds will be deeply sequenced by high-throughtput, to determine the candidate miRNA target mRNAs. The pre-miRNA sequences and the miRNA target genes will be cloned, and the biological functions of these three miRNAs and their target gens will be identified by two approaches, i.e. VIGS of wheat seed-specific gene expression and wheat transgenic overexpression. The main purpose of this study is to unveil the functions of the 3 miRNAs and their regulatory network in wheat seed growth and development process. We also hope to fund miRNA or miRNA target having potential application in quality and high-yield wheat breeding.

英文关键词： wheat;seed;microRNA;functional identification;regultory network