基于体内点击化学的光学纳米探针的构建及其活体成像研究

项目名称： 基于体内点击化学的光学纳米探针的构建及其活体成像研究

项目编号： No.81501591

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 医药、卫生

项目作者： 张鹏飞

作者单位： 中国科学院深圳先进技术研究院

项目金额： 18万元

中文摘要： 分子影像技术在现代诊断学中发挥着越来越重要的作用。光学生物成像技术作为无创可视化成像技术的新方法和手段之一，操作简单、成像迅速，能够直观的监测活体动物体内的基因表达和细胞活动。纳米技术对于分子影像造影剂构建提供了新的机遇。发展无毒的光学纳米探针并将纳米颗粒优越的光学性能与点击化学的特异性结合起来发展能在活体内复杂环境中实现高效稳定标记的纳米探针将为癌症的特异性靶向和早期诊断提供有力帮助，为光学纳米探针的转化医学研究奠定良好基础。本项目将在前期近红外量子点纳米材料及点击化学偶联技术的工作基础上，研发基于无毒近红外量子点及近红外染料的光学纳米探针，进一步结合生物正交化学构建兼具良好生物相容性的可在活体内实现点击化学反应的光学纳米探针，实现对目标细胞的靶向标记，为进一步拓展纳米探针的诊疗应用及临床转化提供有力支持。

中文关键词： 纳米探针；纳米生物医学；活体成像；点击化学；量子点

英文摘要： In vivo medical imaging has made great progress due to advances in the engineering of imaging devices and developments in the chemistry of imaging probes. Molecular imaging is widely considered the future for medical imaging. Optical imaging approaches are emerging as promising high-resolution modalities for the cancer detection. The optical signals can provide the molecular information of biological tissues and are related to the change of some significant physiologic parameters. Advances in nanotechnology have produced significant contributions to the biomedicine. One of the most exciting and rapidly progressing areas of nanotechnology in biomedical research is the development of nanoparticle-based probes for in vivo cancer molecular imaging.The two major concern for nanomaterials as contrast agents and biotags are their toxicity and their specific conjugation ability. In this proposal, we will combine bioorthogonal chemistry, Aggregation Induced Emission and non-toxic quantum dots together to develop biocompatible receptor-targeted near-infrared optical nanoprobes for cancer targeting and imaging. Furthermore, we will try to the potential of as-prepared nanoprobes for translational medicine.

英文关键词： nanoprobe;nanobiomedicine;in vivo imaging;click chemistry;quantum dots