基于谱效关系的龙利叶质量评价物元分析模型的研究

项目名称： 基于谱效关系的龙利叶质量评价物元分析模型的研究

项目编号： No.81460601

项目类型： 地区科学基金项目

立项/批准年度： 2015

项目学科： 医药、卫生

项目作者： 丘琴

作者单位： 广西中医药大学

项目金额： 47万元

中文摘要： 龙利叶Sauropus rostratus Miq. [Sauropus Spatuli folius Beille]为广西民间常用壮药，具有润肺止咳，治疗支气管炎、上呼吸道感染的作用，本课题组研究表明龙利叶具有明显抗炎作用.本项目拟在前期初步对龙利叶质量评价研究的基础上，对龙利叶的安全性检查项目进行研究，并应用中药化学、中药药理学和中药血清药物化学的技术和方法，采用HPLC-DAD-MS追踪分析，建立HPLC化学指纹图谱和血清指纹图谱，获得化学数据，对其抗炎、止咳作用机制进行研究，获得药效数据，用灰关联度法建立龙利叶抗炎止咳作用的谱效关系；将所得的化学数据、药效数据、药效主成分标准化，提取有效特征，用物元分析方法，建立龙利叶质量评价物元分析模型，以在实际应用时，可快速给出龙利叶的药效和质量等级，构建稳定、准确、简便的符合中医药理论的龙利叶质量评价模型，为龙利叶的开发利用，中药材的质量评价提供技术支撑和新的思路。

中文关键词： 龙利叶；抗炎止咳作用机制；谱效关系；物元分析；质量评价

英文摘要： Sauropus rostratus Miq.is a Zhuang medicine in folk of Guangxi province,This herb has efficacies of moistening lung to stop coughing and treating bronchitis and upper respiratory tract infection. Preview research from this project group have showed that the anti-inflammatory action was observed.Based on the preview research on the quality evaluation ,in this study , the examination items will be researched,technologies and methods such as chemistry of Chinese materia medica, pharmacology of traditional Chinese medicine, serum pharmacochemistry and LC-DAD-MS analysing are used to establish HPLC chemical fingerprint and serum fingerprint,which are used as chemical data;the effect data are based on the anti-inflammatory and cough-relieving mechanism research.Based on the chemical data and effect data ,spectrum-effect relationship are established by grey incidence degree method .With the spectrum-effect relationship,the chemical data and the effect data,the quality evaluation model of Sauropus changianus S.Y.Hu are established by matter-element analysis method.The model is stable,accurate ,simple and accorded with traditional chinese medicine theory,which could showed the effect and quality grade of Sauropus rostratus Miq..The project could provied technical support and new thought for the development and utilization of Sauropus rostratus Miq. ,as well as for the quality evaluation of the traditional chinese medicine.

英文关键词： Sauropus rostratus Miq.;Anti-inflammatory and cough relieving mechanism;spectrum-effect relationship;matter-element analysis;quality evaluation