眶内针刺对前部缺血性视神经病变基因表达谱的影响及其网络模块化分析

项目名称： 眶内针刺对前部缺血性视神经病变基因表达谱的影响及其网络模块化分析

项目编号： No.81503453

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 医药、卫生

项目作者： 王影

作者单位： 中国中医科学院眼科医院

项目金额： 18万元

中文摘要： 前部缺血性视神经病变（AION）作为受全身、局部因素均影响较大的临床高发、严重致残、致盲性眼病受到医者的关注。本课题组通过前期临床研究证实针刺治疗AION有效率达61.1%。然而，众所周知，AION发生的病理过程是极其复杂的，单靶点或单通路的研究揭示系统的治疗机制存在瓶颈。后基因组（功能基因组）、基因芯片技术的发展以及复杂生物网络的模块化分析思路为我们的研究提供了曙光。简言之，就是对疾病模型干预后，利用基因芯片技术获得基因表达谱数据，筛选出差异表达基因明确干预靶点，运用计算机构建疾病干预的靶点相关网络，并对该网络进行模块划分及功能注释，从而，明确该治疗方法的功能模块及治疗机制。因此，本课题旨在通过对眶内针刺干预AION基因表达谱的影响及网络模块化分析，明确该疗法对AION多靶点干预机制。多因素致病特点的AION个体与针刺干预多靶点分析的结合，将加速推动针刺疗法在眼科临床中的应用。

中文关键词： 前部缺血性视神经病变；视网膜神经节细胞；针刺治疗；基因芯片；网络模块

英文摘要： Anterior ischemic optic neuropathy (AION) is an eye disease with high incidence of severe disability and blindness affected by systemic and local factor, which are more and more concerned by ophthalmologist. For treating AION, it was demonstrated by our team that effective rate had been up to 61.1% by using acupuncture treatment. However, it is well known that pathological process of AION is extremely complex, which leads to a bottleneck that trying in vain to uncover the treatment mechanism by single target or pathway. Fortunately, the development of post-genome (functional genome) and biochip technology and modular analysis method of complex biological networks may provide a novel paradigm for mechanism comprehension. Briefly, after treating diseased model, we can get the gene expression data by microarray experiment and screen out the differential expression genes for further confirming the therapeutic targets. These targets here are using for constructing the molecular network. For this network, both of module division and module function annotation are aimed at identifying the functional module of treatment and making the therapeutic mechanism clear. Consequently, it is an opportunity for our team to dissect the multi-target intervention mechanism of intraorbital acupuncture in the treatment of AION through analyzing the change of gene expression profiles and network modules. We suggest that combining multifactorial pathology of AION with multi-target intervention analysis of acupuncture may help to promote the application of acupuncture in clinical ophthalmology.

英文关键词： anterior ischemic optic neuropathy;retinal ganglion cell;acupuncture treatment;gene chip;network module