基于纳米金、量子点的均相可视化技术在多组分同步检测中的应用研究

项目名称： 基于纳米金、量子点的均相可视化技术在多组分同步检测中的应用研究

项目编号： No.31472126

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 畜牧学与草地科学

项目作者： 贺平丽

作者单位： 中国农业大学

项目金额： 85万元

中文摘要： 多组分残留检测以其高效快速等优势迅速成为食品检测技术发展的热点。目前多残留检测方法主要是利用大型仪器如色谱质谱仪，测试成本和难度都比较大。而基于化学、免疫学等的快速检测技术虽不需要大型仪器，操作也简单，但只能实现单一组分的检测。因此，本项目提出基于纳米金、量子点的高灵敏度的均相可视化多组分快速检测技术研究，即将纳米金、量子点在不同的尺寸、不同聚集状态所表现出的颜色不同和相应紫外、荧光光谱的差异，与均相反应的简单快速相结合，采用化学比色反应或荧光均相免疫的方法，不需要仪器，只需通过检测样品的溶液颜色变化实现在复杂基质如饲料、牛奶、血液、尿液等中的beta-受体激动剂、四环素类药物、霉菌毒素、大豆抗原蛋白等多组分残留检测。同时借助一些提高灵敏度的检测技术如纳米种子的催化反应等，使灵敏度也能达到甚至超过仪器检测的水平。该技术能够在短时间内分析多个样本的多种成分，真正实现在种类和数量上的高通量。

中文关键词： 多组分；纳米金；量子点；可视化；快速检测

英文摘要： Recently, the simultaneous determination of multiresidues in food and feed is the research hot point. However, these methods are mainly based on the expensitive instruments such as high performance liquid chromatography or liquid chromatography tandem mass spectrometry, which is difficult and expensive. The rapid dectection based on the chemistry or immunology need not large apparatus and equipment, and is easy to operate. But the shortcoming of rapid detection is only dectect a analyte one time. Thus, this project is that the multicomponent simultaneous detect using gold nanoparticle and quantum dots based homogeneous colorimetric assays with the naked eye. For example, beta-agonists showed the capability of directly reducing HAuCl4 into atomic gold, which involved oxidation of the amine or phenol group on the benzene ring of the beta-agonists. The resulted atomic gold formed AuNPs spontaneously, which is red with naked eye and had strong plasmon absorption at 528 nm. The linear relationship between the concentrations of beta-agonists and the AuNPs plasmon absorbance granted quantitative determination of beta-agonists in solutions. In addtion, QDs are robust inorganic fluorophores that exhibit size-dependent fluorescence emission spectra that span the visible spectrum. Their broad emission spectra allow simultaneous excitation of different particle sizes at a single wavelength with emission at multiple wavelengths. Thus, Quantum dots (QDs) have the potential to simplify the performance of multiplexed analysis. Sandwich immunoassays for the detection of multicompent were performed simultaneously in a homogeneous immunosaay using magnetic beads as support. The developed colorimetric assay can be used for the detection of beta-agonists, terracyline antibiotics, bacterial toxins, soybean proteins from feed, serum, urine and other liquid samples in the presence of interference from complex matix, which is a real high throughput rapid method.

英文关键词： simultaneous determination;quantum dots;gold nanoparticles;homogeneous reaction;colorimetric assays