玉米杂种优势转录组新转录本和特异差异表达基因及其调控代谢功能的研究

项目名称： 玉米杂种优势转录组新转录本和特异差异表达基因及其调控代谢功能的研究

项目编号： No.31271793

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 农业科学

项目作者： 季静

作者单位： 天津大学

项目金额： 80万元

中文摘要： 杂种优势的分子机理依然没有完全揭开。了解杂种优势的新转录本(NTs）和特异差异表达基因(DEGs）,不仅对全面揭开杂种优势分子机理有创新性的贡献,还对品质的预测、种质的创新、杂优育种效率的提高有重要意义和应用前景。与国际上其他报道相比(Nature Genet. 2012；2010；Science. 2009等)，我们分析了F1与亲本对环境反应的一系列超亲现象,首次做了杂种优势启动模式的3个转录组测序, 测序长度相当于覆盖经典玉米全基因组3.14倍。本项目对首次获得的257个F1 NTs,865个杂优特异DEGs进行研究, 利用RT-PCR、Real-time PCR、HPLC和Gene ontology等，研究最少有多少NTs和DEGs是杂种优势所必须的基因；它们有什么特殊功能；及对代谢组的优化作用；并通过转基因研究重要基因对优势性状的多重贡献, 最后，创建玉米杂种优势启动基因库。

中文关键词： 转录组；基因调控；新转录本；差异表达；代谢途径

英文摘要： Hybrid vigor, or heterosis, is the phenomenon whereby the progeny of particular inbred lines have enhanced agronomic superior phenotypes and stress tolerance relative to their parents and widely exploited in applied plant breeding. However, the responsible molecular mechanisms have not been determined. Understanding the novel transcripts(NTs）and differential expression genes (DEGs）is essential for studying the complexity of molecular mechanisms of hybrid vigor. Here, we present the first dynamic transcriptomes to switch on heterosis in cultivated maize. Using high-throughput paired-end RNA-seq, we have unambiguously detected 3 dynamic transcriptomes of maize hybrid F1 and both parents for the reaction after treated by NaCl. We sequenced 7,224Gb RNA that 3.14-fold coverage of the classic maize genome, and analyzed 71,364 cDNAs and compared 121 metabolic pathways to study the heterosis. We conducted a comparative analysis of hybrids and parental inbred lines, corresponding a rigorous algorithm showing only 865 DEGs and 257 NTs compared with its parental lines. DEGs and NTs with more significance will be carried out into functional analysis. The NTs and DEGs will be validated by using RT-PCR (reverse transcription-PCR). Furthermore, the genes expression will be detected via the real- time PCR experiments. The metab

英文关键词： dynamics transcriptome；novel transcripts(NTs）；gene regulation；differential expression；metabolic pathway