盐胁迫下鸡爪槭表达谱解析及耐盐关键基因挖掘

项目名称： 盐胁迫下鸡爪槭表达谱解析及耐盐关键基因挖掘

项目编号： No.31300584

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 农业科学

项目作者： 荣立苹

作者单位： 延边大学

项目金额： 23万元

中文摘要： 我国沿海许多城市具有不同程度的盐碱化，具有城市美化功能的耐盐碱树种相当贫乏，因此迫切需要选育耐盐观赏树种和发掘耐盐关键基因。鸡爪槭是世界著名的彩叶观赏树种，但目前还没有可以在盐碱地大面积推广应用的品种。耐盐鸡爪槭材料JB-10-15 的发现，为鸡爪槭耐盐机理研究提供了良好试材。本课题以鸡爪槭耐盐资源JB-10-15 及普通鸡爪槭为主要研究对象，通过高通量测序，运用生物信息学方法对测序数据进行分析，比较鸡爪槭耐盐突变体及野生型的表达谱差异，分析差异表达基因代谢途径，最终筛选出鸡爪槭耐盐的关键基因，并利用实时定量PCR对相关基因进行表达验证。利用同源克隆及RACE 等手段克隆鸡爪槭耐盐的关键基因，通过转化拟南芥等模式植物验证关键基因功能，探索控制其耐盐分子机理，为耐盐新品种选育奠定基础，从而推进槭树科耐盐基因工程育种进程，有利于盐碱地的治理、开发与利用，对于沿海滩涂地区的可持续发展有重大意义。

中文关键词： 鸡爪槭；盐胁迫；测序；差异表达基因；

英文摘要： A great many cities off the coast of China prone to salinization-alkalization, the resources of greening and landscaping were quite poor. With the increase of environmental stress, environmental improvement was gradually noticed. So the salt-tolerant key gene need to be identified. Acer pal matum is a famous color-leaf ornamental plant in the world, but there is no salt-tolerant varieties of Acer palmatum used in saline land in large scale applications. Discovery of Salt-tolerance JB-10-15 supply excellent material for studying mechanism of salt stress in Acer palmatum. Using the Salt-tolerance JB-10-15 and the wild type of Acer palmatum as material, expression profile differences were compared through high-throughput sequencing and bioinformatics, and expressed genes in metabolic pathways were analyzed, eventually the key genes of salt tolerance in Acer palmatum were filtered out. And expression of the important gene will be studied by real-time quantitative PCR. The key genes which control the salt-tolerant will be cloned by using homologous cloning and RACE; and the gene function will be verified by being transformed into model plant. This work can help us master the molecular mechanism of salt- tolerant in Acer palmatum and promote the breeding process by genetic engineering in Aceraceae plants.

英文关键词： Acer palmatum；Salt tolerance；RNA-seq；differentially expressed genes；