基于抗炎活性成分群的中国“杨树型”蜂胶质量评价体系研究

项目名称： 基于抗炎活性成分群的中国“杨树型”蜂胶质量评价体系研究

项目编号： No.31672499

项目类型： 面上项目

立项/批准年度： 2017

项目学科： 畜牧学与草地科学

项目作者： 玄红专

作者单位： 聊城大学

项目金额： 25万元

中文摘要： 蜂胶具有多种药理学活性，但应用中最大的困难就是药效不稳定，药效物质基础不明确，究其原因是蜂胶质量控制体系仍不完善。本课题组前期通过半制备型高效液相色谱（PHPLC）分离纯化了蜂胶水溶物中11种组分，通过不同细胞模型对其活性进行初筛，发现不同组分功效不同，我们推测蜂胶药理学活性不是单一组分发挥功效，而存在一个“活性成分群”。蜂胶发挥不同药理学功效的“活性成分群”是否相同，以及同一药理学活性在不同细胞模型中“活性成分群”是否相同均不明确。本课题将以蜂胶抗炎活性为突破口，在蜂胶固有成分表征的基础上，通过不同的炎症细胞模型，建立“谱-效”关系，明确蜂胶抗炎功效的药效物质基础，并通过在线高效液相色谱结合PHPLC，制备蜂胶抗炎功效的“候选活性成分群”，进而深入发掘蜂胶发挥抗炎功效的“活性成分群”，以期建立以“活性成分群”为标示量的蜂胶抗炎质量控制评价体系，为蜂胶其它活性质量标准的建立提供依据。

中文关键词： 蜂胶；质量控制；抗炎；谱-效关系；活性成分群

英文摘要： Propolis has numerous biological properties for preventing diseases and promoting health. However, the therapeutic effects of propolis are not stable and its material basis is not fully understood. This is because the quality control and evaluation system of propolis needs to be perfected. We previously separated and purified 11 chemical components from water soluble propolis by semi-preparative high performance liquid chromatograph (PHPLC). The biological activities of these components had been preliminarily studied through different cell models, showing that different components had different effects. We speculated that the biological activities of propolis do not depend on a single component, and a “bioacitve components group” might play a key role. It is not clear whether the “bioactive components group” with different pharmacological activities is the same, and whether the “bioactive components group” with the same pharmaceutical activities is the same in different cell models. In this study we will focus on the anti-inflammatory effects of propolis. Based on the natural components screening through different inflammatory cell models, we will establish a spectrum-activity relationship to elucidate the “material basis of pharmaceutical effects” of the anti-inflammatory activity of propolis. Through online HPLC testing together with PHPLC, we prepare and explore the “candidate bioactive components group” of the anti-inflammatory effects of propolis. We aim to establish quality control and evaluation system for propolis which can be marked by “bioactive components group”, and provide basis for other bioactive quality standards.

英文关键词： propolis; quality control;anti-inflammatory;spectrum-activity relationship; bioactive components group