稀土上转换纳米颗粒对间充质干细胞命运的调控

项目名称： 稀土上转换纳米颗粒对间充质干细胞命运的调控

项目编号： No.21501090

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 环境科学、安全科学

项目作者： 任娜

作者单位： 济南大学

项目金额： 25万元

中文摘要： 稀土上转换纳米颗粒（UCNPs）具有优异的光学和化学稳定性、较高的灵敏度和信噪比、以及近红外激发光的组织穿透深度强、零背景荧光干扰和组织损伤等优点，在医疗诊断方面发挥着日益重要的作用。目前UCNPs的细胞相容性和活性的研究仅局限于癌细胞，而对正常细胞行为影响的研究较少，有待进一步深入。间充质干细胞（MSCs）是一类具有多向分化潜能的成体干细胞，在治疗疾病、组织修复和正常生理功能方面发挥着重要作用。本申请项目拟研究不同成分、尺寸、表面配体和浓度的UCNPs对MSCs的影响，通过荧光成像、染色、蛋白检测、基因分析等相关技术，观察UCNPs在MSCs中的分布，分析UCNPs的理化性质对MSCs细胞增殖、多向分化等细胞行为的影响规律，研究其在亚细胞、蛋白和基因水平上的活动及相关的信号通路，明确UCNPs对MSCs命运调控的机理，对生物医用UCNPs的构建及其临床应用具有重要理论意义和实际价值。

中文关键词： 稀土上转换纳米颗粒；间充质干细胞；细胞功能；多向分化；信号通路

英文摘要： Rare earth doped upconversion nanoparticles (UCNPs) have attracted increasing attention in bioimaging and photodynamic therapy (PDT) because of their unique chemical and optical features, including low autofluorescence backgrounds, high signal to noise ratio (SNR), deep penetration depth under near-infrared (NIR) radiation, resistance to photobleaching, and low toxicity. Currently, the biocompatibility studies of UCNPs mainly focused on influence of targeted cancer cells and there still lack , studies of the potential effects on normal cells. Herein, we intend to study the effects of UCNPs on mesenchymal stem cell (MSC), which is a class of pluripotent adult stem cells and play an important role in the treatment of diseases, tissue repair and normal physiological function. The applicant will employ fluorescence imaging, staining, protein assay, genetic technique to study the distribution of UCNPs in MSC and analyze the impact of physicochemical property on cell proliferation, osteogenic, cartilage, adipogenic differentiation and other cell functions. Further study will focus on the interaction and related signal path at subcellular, protein, gene level, define the mechanism about the regulation of UCNPs on MSC. This research will play a solid foundation for the construction and clinical application of biomedical UCNPs system.

英文关键词： rare earth doped upconversion nanoparticles;mesenchymal stem cells;cell function ;multipotential;signal path