黄绿青霉素致血管内皮损伤机制及硒元素的抗损伤作用研究

项目名称： 黄绿青霉素致血管内皮损伤机制及硒元素的抗损伤作用研究

项目编号： No.81202170

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 预防医学、地方病学、职业病学、放射医学

项目作者： 侯海峰

作者单位： 泰山医学院

项目金额： 23万元

中文摘要： 调查显示,在我国从东北-西南的带状分布区内，部分地区心血管病高发。该地区有两个突出环境问题：1.黄绿青霉素（CIT）的粮食污染较为严重；2.饮食中硒元素（Se）不足。最近研究发现CIT可引起内皮细胞凋亡和功能异常。血管内皮覆盖于血管腔内，为血液流动提供光滑的内表面，并具有重要的血管调节功能，内皮损伤是动脉粥样硬化等血管疾病的起点，另外Se有减轻内皮损伤的作用。以上研究提示CIT可能通过对内皮的损伤引发血管疾病，但有关CIT对内皮损伤和Se对该损伤的保护作用的研究尚不深入、对损伤的机制并不清楚，且该损伤能否导致动脉粥样硬化还缺乏证据支持。本项目旨在通过人脐静脉血管内皮细胞原代培养和动物实验两个途径，探索CIT引发内皮凋亡的机制，阐明核因子-κB在CIT引发内皮功能损伤过程中的调控作用，筛选内皮损伤标志物，发现CIT导致动脉粥样硬化的证据，研究Se对CIT所导致的内皮损伤的保护作用。

中文关键词： 黄绿青霉素；硒；血管内皮细胞；动脉粥样硬化；毒性

英文摘要： The mortality rates of cardiovascular disease are not alike in different areas with the characteristics of distinct spatial distribution in the world, and this characteristics contributes to the analysis of the pathogenesis of the disease. The mortality rate of cardiovascular disease is higher in the zonal distribution area from Northeast of China to Southwest than any other regions of the country. There are two obvious environmental problems in the area. Firstly, the pollution of citreoviridin (CIT) in food is serious. Secondly, the intake of selenium (Se) in food is low. The recent research indicates that the mycotoxin of CIT is produced by some fungi such as Penicillium Citreoviride. We find that CIT induced cell apoptosis and cell dysfunction when cultured with vascular endothelial cells. Vascular endothelial cells constitute endothelium in the surface of vessel lumen, and play important roles in regulating physiological function of vessel. The dysfunction of vascular endothelium is the leading cause of atherosclerosis and results in cardiovascular disease. Therefore it is supposed that CIT induces cardiovascular disease via impairment of vascular endothelial cells. In addition, as Se has protective effect on vascular endothelial cells preventing from impairment, the deficiency of Se may relate to cardiovasc

英文关键词： citreoviridin(CIT)；selenium；vascular endothelial cell；atherosclerosis；toxicity