樟疫霉致病性相关GPCR-PIPK鉴定与机理研究

项目名称： 樟疫霉致病性相关GPCR-PIPK鉴定与机理研究

项目编号： No.31500526

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 农业科学

项目作者： 戴婷婷

作者单位： 南京林业大学

项目金额： 20万元

中文摘要： 樟疫霉引起的病害是农林生产上的严重病害，对农田、森林、园艺等经济体系造成巨大影响。G蛋白偶联受体(G protein coupled receptor, GPCR)和磷脂酰肌醇磷酸激酶（phosphatidylinositol phosphate kinase, PIPK）是两类参与不同途径的信号传导通路，对物种的生长发育阶段起着重要调控作用。本项目从樟疫霉全基因组数据库中挖掘出大量GPCR-PIPK候选编码基因，采用Real-time PCR 分析候选基因的转录表达模式，在此基础上利用瞬时沉默体系和稳定转化体系筛选出致病性相关的GPCR-PIPK，明确致病性相关GPCR-PIPK候选编码基因的生物学功能，同时鉴定其调控的下游基因的信号通路，阐明樟疫霉的生长发育和致病等重要生理过程中的作用机制，为新型杀菌剂的研发提供可能的分子靶标，从而为有效防控樟疫霉引起的病害提供理论依据。

中文关键词： 致病基因；受体蛋白；基因沉默；樟疫霉

英文摘要： The genus Phytophthora cinnamomi causes important diseases in ornamental, agronomic and forest ecosystems worldwide. G protein coupled receptor (GPCR) and phosphatidyl inositol phosphate kinase (PIPK) are responsible for transducing extracellular signals into intracellular responses that involve complex intracellular-signaling networks, affecting the development and metabolic pathway. In this project, we predicted many putative GPCR-PIPK coding genes in the genome of P. cinnamomi according to the sequences and domains of GPCR reported in other organisms. The ortholog alleles and the gene genomic distribution patterns of the putative GPCR-PIPK encoding genes of P. cinnamomi were analyzed. The transcription profile of putative GPCR-PIPK was also performed by RNA-sequencing and qRT-PCR. The stable transformation was used to generate silenced transformants. Taken together, our results indicate essential roles of GPCR-PIPK candidate genes in development and pathogenicty in P. cinnamomi. Based on current researches, this project will further identify the downstream genes involved in GPCR-PIPK using RNA-sequcing and analyze the gene function and elucidate the regulating mechanisms. Results of this project will provide molecular targets for novel fungicide development and scientific basis for strategy of controlling Phytophthora root rot disease by P. cinnamomi.

英文关键词： pathogenic genes;receptor protein;gene silencing;Phytophthora cinnamomi