青钱柳多糖CPP-1的精细结构及其抗肿瘤活性构效关系

项目名称： 青钱柳多糖CPP-1的精细结构及其抗肿瘤活性构效关系

项目编号： No.31201297

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 食品科学、农学基础与作物学

项目作者： 谢建华

作者单位： 南昌大学

项目金额： 24万元

中文摘要： 多糖是一类重要的生物信息分子，是基因的信息延续，现已证明多糖具有显著的抗肿瘤生物活性，但其活性功能的分子基础、机理及构效关系尚不清楚。本项目以从我国特色资源青钱柳中分离纯化出的具有抗肿瘤活性的多糖CPP-1 为研究对象，采用红外光谱、激光拉曼光谱、色谱质谱联用、核磁共振谱等现代仪器分析方法，研究其理化性质，分子量、纯度，单糖、糖醛酸、氨基酸等精确组成，采用完全酸水解方法、X-射线和圆二色谱等研究其糖链精细结构如主链和支链的组成、糖残基间的连接顺序、连接方式及糖链释放等，从而解析其精细结构，并结合细胞生物学与分子生物学方法研究多糖对HeLa细胞凋亡、caspase-3 mRNA 及caspase-3 等蛋白表达的影响，从基因水平、蛋白水平探讨CPP-1 诱导HeLa细胞凋亡的作用机理，在此基础上探讨其抗肿瘤活性的构效关系。研究结果为植物多糖抗肿瘤活性构效关系的系统研究提供重要的方法学参考。

中文关键词： 青钱柳；多糖；构效关系；生物活性；机理

英文摘要： Polysaccharide is an important biomolecules which continue the genic information.In recent years, it has been demonstrated that polysaccharide has significant antitumor activities both in vivo and in vitro. However,the molecular basis, mechanisms of these activities and the structure-activity relationship of polysaccharide are still unknown. Based on the previous work which has extracted and purified an antitumor polysaccharide of CPP-1 from Cyclocarya paliurus (Batal.) Iljinskaja, the present study is trying to analyze the physicochemical properties, molecular weight and chemical composition (including the monosaccharide, uronic acid and amine acid composition) of CPP-1 with modern instrumental analysis. In addition, the fine structure of CPP-1 is going to be studied with completely acid hydrolysis, X-ray, and circular dichroism (CD) to elucidate the backbone and side chain composition, the order of connection among sugar residues and the release of sugar chain. Furthermore, the antitumor effects of CPP-1 will be evaluated on the model of HeLa cell, and assessed by the expression of caspase-3 mRNA and caspase-3 protein. Thus，the mechanism of inducing cell apoptosis will be discussed at genetic and protein level with cell biology and molecular biology methods，and the relationship between structure and antitumor

英文关键词： Cyclocarya paliurus (Batal.) Iljinskaja；polysaccharide；structure-activity relationship；bioactivity；mechanism