Twist基因在虾夷扇贝肌肉生长发育中的时空表达模式及调控机制研究

项目名称： Twist基因在虾夷扇贝肌肉生长发育中的时空表达模式及调控机制研究

项目编号： No.31502162

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 农业科学

项目作者： 焦文倩

作者单位： 中国海洋大学

项目金额： 20万元

中文摘要： 闭壳肌重量是扇贝重要的经济指标，已有关于扇贝闭壳肌生长的研究主要集中在肌肉生长相关个别基因，对于贝类肌肉发生及生长的整体调控通路目前尚不清晰。Twist基因是无脊椎动物胚胎中胚层分化和肌肉原基形成的关键起始转录因子，并对维持成体肌肉干细胞的存在起重要作用。我们在前期研究中发现Twist基因在扇贝成体横纹肌中特异高表达，暗示Twist基因与扇贝肌肉生长发育相关，但是有关Twist基因参与的调控通路目前在扇贝中还不明确。本项目将在此基础上开展虾夷扇贝Twist基因在胚胎发育和成体生长中的时空表达特征分析，构建Twist相关基因共表达网络并筛查与其互作的蛋白，初步揭示Twist基因在扇贝肌肉生长中的调控作用。项目将解析虾夷扇贝Twist基因的时空表达模式及其在肌肉生长中的调控网络，以期深入理解无脊椎动物的肌肉发生、生长调控机理，为Twist基因在高产扇贝育种中的应用价值提供理论参考。

中文关键词： 虾夷扇贝；Twist基因；肌肉生长；时空表达；共表达网络

英文摘要： The weight of adductor muscle is an importantly economic indicator in scallops. Previous researches about muscle growth mainly focused on several candidate genes. However, regulatory pathways of myogenesis and muscle growth are not very clear. Twist gene is a key initiation transcription factor in mesoderm formation and myogenesis in invertebrates, which is expressed in muscle precursors of adult muscle. Recently we found that in Yesso scallop, the expressional level of Twist gene was specifically high in striated muscle of adult individuals, compared with other adult organs, which indicated that Twist gene was related of muscle growth of scallops. Based on this previously interesting result, our project will characterize spatio-temporal expression pattern of Twist gene during embryo developmental process and in the adult organs of Yesso scallop, study its interacting genes and gene coexpression network related with muscle growth, and analyze regulatory function of Twist gene related with scallop muscle growth. The results of our project will be helpful to improve our understanding in genetic mechanism of invertebrate muscle growth regulation and also benefit the molecular breeding of scallop aiming to growth improvement.

英文关键词： Yesso scallop;Twist gene;muscle growth;spatio-temporal expression;coexpression network