共轭亚油酸降低草鱼肝脏脂肪积累关键调控因子的筛选与鉴定

项目名称： 共轭亚油酸降低草鱼肝脏脂肪积累关键调控因子的筛选与鉴定

项目编号： No.31302195

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 农业科学

项目作者： 董桂芳

作者单位： 武汉轻工大学

项目金额： 24万元

中文摘要： 营养性脂肪肝是制约水产养殖业可持续发展的瓶颈问题，目前尚无有效的防治措施。在我们发现共轭亚油酸(CLA)可有效降低草鱼肝脏脂肪积累的基础上，本项目拟以草鱼为研究对象，通过生长实验，确定CLA降低其肝脏脂肪含量的适宜添加量(CLAOPT)，随后综合运用转录组测序、分子和免疫技术，系统地对CLA发挥降脂功效的关键调控因子展开研究。通过对CLAOPT组和未添加CLA组肝脏的比较转录组研究，经转录过程中差异表达基因的细胞学功能和机体代谢调控网络分析，筛选出关键调控因子；继而通过mRNA和蛋白表达量的双重鉴定得到若干关键调控因子，为阐明CLA发挥降脂功效的分子机理奠定研究基础。本项目的实施将会为通过CLA营养调控防治草鱼营养性脂肪肝并选育CLA降脂作用敏感基因型鱼类奠定理论基础，拓宽鱼类营养性脂肪肝防治的研究视角，并为开发CLA类饲料添加剂和保健水产品以及缓解现阶段紧张的饲用鱼油资源提供理论依据。

中文关键词： 草鱼；瓦氏黄颡鱼；共轭亚油酸；脂肪积累；转录组测序

英文摘要： Nutrition-associated liver disease (NALD) is one of the bottleneck problems for a sustainable development of aquaculture. However, there is no known effective prophylaxis for NALD, and there is no effective treatment for most cases of NALD. It has been revealed that dietary conjugated linoleic acid (CLA) can effectively decrease liver lipid concentration in grass carp in our pre-experiment. The present study aims to clarify the key regulators for this lipid-lowering effects of CLA in grass carp. In the present study, an 8 weeks of feeding trial was conducted to identify the optimal dose of CLA for grass carp (CLAOPT), and the comparative transcriptome were conducted in livers of the CLAOPT and no CLA-supplemented groups to obtain the differentially expressed genes, and then the cellular function of these differentially expressed genes and their whole-body metabolic pathways were analyzed to obtain the key regulators in the transcriptional regulation networks. Moreover, qRT-PCR and immunohistochemistry will be used to identification those key regulators in their transcriptional and translational regulation and finally, to gain the key regulators for the fish. The present study will be essential for further elucidating the molecular mechanism and for effective prophylaxis and treatment of the NALD in grass carp, a

英文关键词： grass carp；darkbarbel catfish；conjugated linoleic acid；lipid accumulation；RNA-seq