木质素在H-LAT预处理中的解构行为及酶解抑制机制研究

项目名称： 木质素在H-LAT预处理中的解构行为及酶解抑制机制研究

项目编号： No.31500491

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 林学

项目作者： 赵超

作者单位： 浙江农林大学

项目金额： 20万元

中文摘要： 木质素在纤维素乙醇制备过程中的酶解抑制作用已得到广泛认可。但是，现行的碱预处理酶解抑制作用仅关联于木质素去除率：①无法表达木质素的基本特征、结构单元及连接键型在预处理中的变化；②无法考察木质素结构单元及连接键型对酶解的抑制机制。本项目拟针对预处理对木质素解构行为及酶解抑制机制的关键科学问题展开研究，以过氧化氢液氨联合预处理（H-LAT）为研究对象，组合运用光谱、波谱、色谱等分析技术定量描述从原材料和预处理底物中提纯的磨木木质素；以其基本特征、结构单元与连接键型的变化为中间载体，一方面研究预处理对木质素结构的影响，解译木质素在预处理中的解构行为，另一方面研究木质素对酶活、酶吸附、酶解速率及酶解率的影响规律，揭示木质素结构单元与连接键型对酶解的抑制机制；进而构建木质素解构行为与酶解率的关联模型，为实现预处理对底物木质素结构的定向调控、提高木质纤维原料的酶解率提供理论依据。

中文关键词： 纤维素乙醇；预处理；木质素；纤维聚糖；抑制机制

英文摘要： Enzyme inhibition of lignin during the process of bioethanol production from lignocellulosic material has been widely recognized. However, the enzyme inhibition of current alkali pretreatment was only associated with lignin removal rate, this was unable to express the changes of composition and structure of residual lignin during pretreatment, and it was unable to reveal the inhibition mechanism caused by composition and structure of lignin. This project is aiming at the effects of pretreatment on lignin structure and inhibition mechanism, and focusing on combined hydrogen peroxide and liquid ammonia treatment (H-LAT). Milled wood lignins isolated from raw materials and pretreatment substrates will be described quantitatively based on the lignin monomer model using spectroscopy, wave spectroscopy, chromatography and other analytical techniques. Based on the changes of properties, composition and structure of lignin, on the one hand, the deconstruction of lignin in H-LAT pretreatment will be interpreted through the study on the effects of pretreatment on lignin structure, on the other hand, the enzymatic inhibition mechanism of lignin will be revealed through the study on the effects of lignin on enzyme activity, enzyme adsorption, hydrolysis rate and enzymatic digestibility. And then to build a relational model between lignin deconstruction and enzymolysis macro results. The results will provide theoretical basis for the control of lignin structure for pretreatment improving the enzymatic hydrolysis of lignocellulose.

英文关键词： Cellulosic ethanol;Pretreatment;Lignin;Cellulosan;Inhibition mechanism