水稻热激反应调控因子-HsHS的功能和机理研究

项目名称： 水稻热激反应调控因子-HsHS的功能和机理研究

项目编号： No.31371557

项目类型： 面上项目

立项/批准年度： 2014

项目学科： 食品科学、农学基础与作物学

项目作者： 孙新立

作者单位： 福建农林大学

项目金额： 79万元

中文摘要： 高温热害对水稻的危害非常严重。我们发现一个新的水稻WRKY转录因子-HsHS，调控高温诱导的细胞程序性死亡。另外，HsHS还受多种生物和非生物胁迫因子以及茉莉酸（JA）的诱导,这些暗示它可能是一个处于调控网络中关键节点的多功能转录因子，在水稻的抗病和抗逆反应中都起着重要作用。本研究拟以野生型、超表达和抑制表达转基因植株为材料，采用高温等环境逆境或不同病原菌接种处理,利用RNA-seq、ChIP-Seq和酵母双杂交等技术，揭示该转录因子的功能及其调控机制，研究结果对水稻热激等逆境反应机理的阐明以及水稻的改良具有重要的意义。

中文关键词： 水稻;热激反应;功能;调控机理;胁迫反应

英文摘要： Heat stress is severely harmfulto the rice growth and production. A novel rice WRKY transcription factor (HsHS) has been identified to regulate programmed cell death induced by high temperature and to respond to several biotic/abiotic stresses and JA. These results imply that HsHS plays a crucial role in the heat shock response. HsHS may be a multi-functional factor, and play an important role in the disease resistance and/or other abiotic stresses too. In order to prove our hypothesis, and reveal the gene function and regulation mechanism, transgenic plants with depressed or overespressed HsHS gene were established, and the genes directly regulated by HsHS were being analyzed with RNA-seq and ChIP-seq, and the proteins that interacted with HsHS will be discovered by screening the Yeast Two-Hybrid library. The result will significantly contribute to further our knowledge about the rice responses to stress and rice improvement.

英文关键词： rice;heat shock;function;regulation mechanism;stress response