大气压等离子体诱导癌细胞凋亡的分子机制研究

项目名称： 大气压等离子体诱导癌细胞凋亡的分子机制研究

项目编号： No.11275261

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 数理科学和化学

项目作者： 杨思泽

作者单位： 厦门大学

项目金额： 72万元

中文摘要： 当前，大气压等离子体在凝血、慢性伤口愈合、牙齿根管治疗、病菌的灭活、癌细胞处理等方面具有广阔的应用前景。但是在理论和应用方面，仍存在等离子体反应粒子较难诊断、放电机制尚不明确，癌症的临床治疗困难等问题。针对这一现状，我们使用大气压等离子体放电装置对处于体表的、适合操作与临床早期处理的皮肤癌与口腔癌细胞进行实验研究。本项目拟利用快脉冲高压探头、ICCD相机、高分辨光谱仪等仪器分析在高压电源驱动下产生惰性气体等离子体羽的时空动力学特性；研究各种不同的反应气体对放电特性（李萨如图形、电子密度、等离子体"子弹"速度等）的影响；找到等离子体放电条件与细胞凋亡、坏死之间的关系。在此基础上，探讨等离子体中各种活性物种与癌细胞之间相互作用的机制，为等离子体生物医学的发展、应用提供理论和实验基础。

中文关键词： 癌细胞；DNA；碱基；OH自由基；

英文摘要： Recently，there has been growing interest in developing their counterparts at atmospheric pressure plasma, particularly for biomedical applications such as coagulation, chronic wound healing, dental root canal treatment, the bacteria inactivation, cancer treatment. However, in theory and applications, there are still many problems, for example, plasma diagnose, discharge mechanism and cancer clinical treatment. In response to this situation, skin and oral cancers in the body surface, which are suitable for operation with the early clinical treatment, are treated by atmospheric pressure plasma. In this study, the fast pulsed high-voltage probes, ICCD camera, and high-resolution spectrometer instrument will be used to analyze spatial and temporal dynamics characteristic of plasma plume driven by the high-voltage power. The discharge characteristics (Lissajous graphics, electron density, plasma "bullet" speed, etc.) for different reaction gases are examined. We will find the relationship between discharge conditions and apoptosis and necrosis. On these bases, we will explore the mechanism of the interaction between the various plasma reactive species and cancer cells, which can supply the theoretical and experimental basis for the development of plasma biomedical applications.

英文关键词： Cancer cell；DNA；Ribonucleoside；OH radical；