FUT8介导糖基化修饰在乳腺癌侵袭转移中的作用及早期诊断价值研究

项目名称： FUT8介导糖基化修饰在乳腺癌侵袭转移中的作用及早期诊断价值研究

项目编号： No.81202084

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 肿瘤学2

项目作者： 岳丽玲

作者单位： 齐齐哈尔医学院

项目金额： 23万元

中文摘要： 糖基化是最常见的翻译后修饰反应之一，岩藻糖基化修饰是肿瘤发生发展中最重要的糖基化形式。然而核心岩藻糖基化修饰在乳腺细胞恶性变过程中所发挥的具体生物学功能尚不清楚。我们前期工作证实，α-1,6岩藻糖基转移酶（FUT8）过表达不仅可以促进乳腺癌细胞的增殖，而且能增加乳腺癌细胞的黏附及迁移能力，提示其在促进肿瘤的侵袭转移上可能发挥着重要作用。本项目拟在此基础上，从细胞学、组织学、血清学等多层次入手，利用基因转染、siRNA等手段调控核心岩藻糖基化修饰水平，探讨核心岩藻糖基化修饰对乳腺癌细胞侵袭转移能力的影响及其调控机制；并从组织学水平揭示FUT8和核心岩藻糖基化修饰的临床意义；最后通过对乳腺不典型增生、原位癌、浸润性乳腺癌患者血清的核心岩藻糖化水平分析，评估血清核心岩藻糖检测对乳腺癌早期临床诊断的价值，为寻找乳腺癌直接的诊断标志物及潜在的治疗靶标，最终实现乳腺癌的早期诊治提供理论和实验依据。

中文关键词： 乳腺癌；核心岩藻糖基化；转移；α-1;6岩藻糖基转移酶；预后

英文摘要： Glycosylation is one of the most common posttranslational modification reactions. Fucosylation is one of the most important glycosylation form in the course of tumorous development. However, we have not still understand what biological function of core fucosylation bring into play in the cells malignant transformation of human breast cancer. Our researchs early days have shown the overexpression of α-1,6 fucosyltransferase (FUT8) have not only promote the proliferation of breast cancer cells, but also could increase adhesion and migration ability abilities of breast cancer cells, suggesting that FUT8 plays an important role in the invasion, metastasis of tumor. Regard these as foundation, starting with cytology, histology, serology and other multi-level, this project will take advantage of gene transfection, siRNA and other means to adjust the level of core fucosylation, discussing effect and its adjustive mechanism of core fucosylation on invasion and metastasis abilities ability of breast cancer cells. Meanwhile, we will reveal the clinic meaning of FUT8 and core fucosylation from the level of histology. Finally, by analyzing serum core fucosylation level of atypical hyperplasia of mammary gland, carcinoma in situ and invasive breast cancer patients, we will appraise serum core fucosylation detection value to

英文关键词： breast cancer；core fucosylation；metastasis；α1;6-Fucosyltransferase；prognosis