玉米ZmSNAC1基因调控植物耐旱的机制研究

项目名称： 玉米ZmSNAC1基因调控植物耐旱的机制研究

项目编号： No.31501314

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 食品科学、农学基础与作物学

项目作者： 卢敏

作者单位： 北京农学院

项目金额： 20万元

中文摘要： NAC转录因子是植物特有的具有多种生物学功能的转录调控因子，因其在抵抗逆境、调节植物生长发育等方面发挥的重要作用，近年来受到研究者的广泛关注，特别在模式植物如拟南芥中NAC家族成员的研究已取得了丰硕成果，但NAC基因的抗逆调控机制及其在重要粮食作物玉米中与抗逆相关的功能研究较少。干旱是影响我国玉米生产、造成粮食减产的重要限制因素之一。目前，玉米中NAC基因耐旱功能的研究仍处于起步阶段，玉米NAC基因调控其耐旱性的作用机制尚不清楚。本研究将进一步明确ZmSNAC1在玉米中的耐旱生物学功能，筛选并鉴定ZmSNAC1上游调控因子和互作蛋白，分析其在干旱和ABA胁迫下的诱导表达模式；明确ZmSNAC1转录调控机制和蛋白互作关键氨基酸序列；分析干旱胁迫下超表达ZmSNAC1株系、RNAi和对照株系的差异表达基因，鉴定其靶基因及生物学功能，探讨ZmSNAC1介导的耐旱分子调控机制，创制耐旱玉米新种质。

中文关键词： 玉米；NAC基因；耐旱；分子机制

英文摘要： NAC transcription factors are plant-specific regulators that have multiple biological functions including stress adaptation and tolerance, plant development, morphological formation and hormone regulation. Recently, NAC transcription factors have received more attention for the great achievements in research on the functions of NAC members in model plants, especially in rice and Arabidopsis. However, the regulating mechanisms of plant stress tolerance mediated by NAC transcription factors, and the functional analysis of maize NAC members in response to abiotic stresses have not been characterized. Drought is one of the major environmental stresses that adversely affect the growth and productivity of maize. At present, the roles and regulatory mechanisms of maize NAC genes in plant drought stress tolerance were still unknown. In this study, we will explore the upstream regulators and interacting protein of ZmSNAC1, and analyze its expression pattern under various stress treatments including drought and ABA. The key region of ZmSNAC1 that involved in protein interaction will be also identified. We will further employ RNA-Seq analysis to identify the differences in gene expression between the ZmSNAC1 overexpression lines, the RNAi lines and the wild type lines under drought stress conditions. In addition, we will characterize the different expressed genes for its important role in stress tolerance. Together, this work will contribute to analyze the drought tolerance mechanism of ZmSNAC1 gene in maize and will be beneficial in molecular breeding to improve crop stress tolerance.

英文关键词： maize;NAC gene;drought stress tolerance;molecular mechanism