棉花BI-1通过调控细胞死亡介导耐逆的研究

项目名称： 棉花BI-1通过调控细胞死亡介导耐逆的研究

项目编号： No.31501351

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 食品科学、农学基础与作物学

项目作者： 张景霞

作者单位： 山东省农业科学院

项目金额： 20万元

中文摘要： BI-1（Bax inhibitor 1）是胁迫信号传导网络中的汇集点，是调控胁迫诱发的细胞死亡的关键因子。但植物中BI-1如何应答胁迫信号来调控细胞死亡尚不清楚。. 已在陆地棉根cDNA文库中克隆到两个BI-1基因，分别命名为BI-1A和BI-1B。研究结果表明，BI-1A和BI-1B的表达量分别在NaCl及黄萎病菌胁迫下上升，暗示它们具有耐受不同类型胁迫的功能。本项目拟在详细研究棉花BI-1A、B时空表达模式及响应胁迫类型的基础上；在棉花中过量或抑制这两个基因的表达，分析它们参与耐逆及对细胞死亡的调控功能；进一步解析BI-1A、B响应不同胁迫信号，调控细胞死亡的可能信号通路。本项目将拓宽对植物通过调控细胞死亡介导耐逆的分子机制的认识，阐明棉花BI-1对不同胁迫信号进行响应的机制，并期望为挖掘、应用能够提高植物广谱耐逆性的基因资源提供理论基础。

中文关键词： 棉花；抗逆性；转基因；功能分析；分子机制

英文摘要： BI-1(Bax inhibitor 1) is the point of stress signal transduction network, and also acts as a key regulator protein conrtolling the cell death induced by various stresses. However, how BI-1 response to stress signals and the role in mediating cell death is still unknown in plant...We have isolated two BI-1 genes from the cDNA library from the upland cotton, and named them BI-1A and BI-1B，respectively. The transcription level of the two genes appear different characters under NaCl and Verticillium Wilt stresses, suggesting that cotton BI-I genes have diverse functions in response to different types of stress. This project will intend to clone BI-1 genes (BI-1A and BI-1B) from upland cotton, and analyze the expression patterns under different stress conditions as the basal study. Then, we will upregulating and inhibiting the expression of the two genes in cotton and Arabidopsis, study their regulator function in cell death caused by stresses and the protective role in plant. Finally, we will clone the promoters of the two genes, and explore the molecular mechanism of how BI-1A and BI-1B response to different stresses and the signal transduction pathway. This project will shed light on the mechanism that how cell regulate cell death caused by stress to adjust the tolerance in plant, and the possible function of BI-1 in cotton in the control of cell death. We expect that this project will the provide gene resource and the theory evidence for improve the broad spectrum tolerance in plant.

英文关键词： cotton;stress tolerance;gene transduction;function analysis;molecular mechanism