金针菇改善记忆功能成分解析及其作用机理研究

项目名称： 金针菇改善记忆功能成分解析及其作用机理研究

项目编号： No.31272220

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 农业科学

项目作者： 胡秋辉

作者单位： 南京财经大学

项目金额： 85万元

中文摘要： 金针菇是我国消费最多的食用菌之一。研究和报道金针菇具有改善记忆的功能，但是金针菇中改善记忆的化学成分和作用机制至今尚未明确。本研究以动物实验模型追踪分离纯化金针菇中改善记忆功能成分，并采用现代波谱技术和化学分析方法解析活性成分的分子结构；通过检测小鼠海马、大脑皮层、丘脑、纹状体等脑组织中的Connexin 36、p-ERK1/2和p-CaMKⅡ蛋白表达，乙酰胆碱、5-羟色胺、多巴胺和去甲肾上腺素神经递质含量，乙酰胆碱酯酶、胆碱乙酰转移酶神经调节酶类和过氧化物酶、超氧化物歧化酶等抗氧化酶类的活性，以及神经细胞间的连接与通讯功能、神经递质信号分子调节与转导作用，阐明金针菇功能成分改善记忆力的分子作用机制，为金针菇功能成分改善记忆力的作用提供理论依据。

中文关键词： 金针菇；改善记忆和学习能力；机制；多糖；黄酮

英文摘要： Flammulina velutipes is one of the most popular mushrooms and has been reported to be beneficial in learning and memory ability. However, the mechanism underlying the improvement of learning and memory components remains unclear. Therefore, animal behavior test was used in the separation and purification of learning and memory improving components from Flammulina velutipes. Spectroscopic and chemical analysis methods were used to characterize the active components in the mushroom. To clarify the mechanism of improving learning and memory ability, effects of active component on the mice biochemical and neurological changes in hippocampus and cerebral cortex, thalamencephalon, and corpus striatum were investigated. This was achieved by determining, expression of Connexin 36, p-ERK1/2 and p-CaMK II, contents of acetylcholine, 5-hydroxytryptamine, dopamine, and norepinephrine and activities of acetylcholinesterase, acetylcholine acetyltransferase, superoxide dismutase, and glutathione peroxidase. The in vitro effects of active components on the gap junctional intercellular communication, neurotransmitters' modulation and signal transduction of neuron cells were also investigated. The results of this research will lay a theoretical foundation for improving learning and memory functions of Flammulina velutipes.

英文关键词： Flammulina velutipes；Improving learning and memory；mechanism；Polysaccharides；Flavonoids