理气化痰活血方药对大鼠冠脉微循环障碍的影响及分子调控机制研究

项目名称： 理气化痰活血方药对大鼠冠脉微循环障碍的影响及分子调控机制研究

项目编号： No.81202799

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 医学八处

项目作者： 王贤良

作者单位： 天津中医药大学

项目金额： 24万元

中文摘要： 在所有与冠脉微循环障碍相关的心脏疾病中，心脏X综合征（又称为微血管性心绞痛）最具代表性，研究认为冠脉微血管内皮功能障碍（CMVD）是其主要原因。我们前期研究发现，气滞痰阻血瘀是心脏X综合征的主要病机特征，据此提出了理气化痰活血法治疗心脏X综合征的假说并得到了初步临床验证。 本研究拟采用经主动脉根部注射月桂酸钠方法建立大鼠冠脉微循环障碍模型，模拟临床患者发作心绞痛而大冠脉无有意义狭窄的情况。考察理气化痰活血方药对大鼠冠脉微血管血栓形成的影响；评价理气化痰活血方药对血内皮素（ET-1）、一氧化氮（NO）、血管性假血友病因子（vWF）及心脏组织中纤维介素（fg12）凝血酶原基因表达的良性调控作用；基于内皮损伤介导的内、外源性凝血和非经典的凝血因子途径，探讨理气化痰活血方药抑制冠脉微血管血栓形成、保护内皮功能的机制。

中文关键词： 冠脉微循环障碍；理气化痰活血方药；微血管血栓形成；分子机制；

英文摘要： Cardiac Syndrome X (CSX), also known as microvascular angina, is the most representative among all coronary microcirculation dysfunction related heart diseases. Studies have shown that coronary microvascular dysfunction, CMVD is the primary cause of the disease but the pathogenesis is not yet fully understood. In our preliminary study, we have discovered that qi, blood and phlegm stagnancy is the main characteristics of the Traditional Chinese Medicine (TCM) pathogenesis. Thus, we proposed the hypothesis of using eliminating phlegm, promoting qi and blood circulation therapeutic method for the treatment of CSX and have proven it in preliminary clinical trials. Our study plans to use aortic root injection of sodium laurate to induce rat models with microcirculation dysfunction to simulate patients with angina but without significant coronary stenosis. This is to investigate the effects and molecular regulatory mechanism of phlegm elimnating, qi and blood circulation promoting therapeutic method on rats with coronary microvascular thrombosis. Positive regulatory effects of the therapeutic method on Endothelin-1(ET-1), nitric oxide (NO), von Willebrand factor (vWF) and the prothrombin gene expression in cardiac tissue fg12 will be assessed. We will also study the molecular biology mechanism of the therapeutic m

英文关键词： coronary microcirculation；qi-regulating phlegm-resolving and blood-promoting；coronary microvascular thrombosis；molecular mechanism；