国家保种动物天祝白牦牛寄生蠕虫分布及分子分类学研究

项目名称： 国家保种动物天祝白牦牛寄生蠕虫分布及分子分类学研究

项目编号： No.31460659

项目类型： 地区科学基金项目

立项/批准年度： 2015

项目学科： 水产学、兽医学

项目作者： 孙晓林

作者单位： 甘肃农业大学

项目金额： 50万元

中文摘要： 天祝白牦牛是甘肃省特有的宝贵畜种资源，属国家保种动物，也是当地牧民必需的生活、生产资料。寄生虫病对其危害十分严重，阻碍着白牦牛产业的持续发展，影响当地经济的增长；且有关白牦牛寄生蠕虫种群的分布和分子分类学研究在国内外未见报道。 本项目拟在甘肃省天祝藏族自治县的不同地理位置、不同季节，选择不同年龄的白牦牛，采用蠕虫完全剖检法，研究其寄生蠕虫的种群分布、分布模式及感染动态，并确定不同年龄白牦牛感染蠕虫的优势种群；采用分子分类方法，就传统方法定种有困难的虫株，对其核糖体DNA的ITS序列进行测定，确定其分类地位。对不同种群牦牛寄生的优势蠕虫虫株的mtDNA遗传标记序列进行分析，以确定其进化关系。预期成果将填补寄生虫种群分布的空白，为天祝白牦牛乃至整个牦牛群体的寄生虫病防治提供科学依据。

中文关键词： 天祝白牦牛；蠕虫；种群分布；分子分类；遗传变异

英文摘要： Tianzhu White Yak is a unique and valuable livestock resource in Gansu Province. It is also the necessary means of subsistence and means of production by local herders. However, parasitic helminths cause significant economic losses and hinder the sustainable development of Tianzhu White Yak industry. There have seen no reports of population distribution and molecular identification of parasitic helminths in Tianzhu White Yak. This project will study the population distribution and infection dynamics of parasitic helminths in Tianzhu White Yak in Tianzhu Tibetan Autonomous Region, Gansu Province by traditional parasitological and molecular approaches. Helminths of Tianzhu White Yak of different age groups from different geographic locations will be collected in different seasons, identified and the dominant species of parasitic helminthes in different ages Tianzhu White Yak will be determined. The internal transcribed spacer sequences of ribosomal DNA from the helminths whose identity could not be determined by traditional parasitological approach will be determined and used to identify their taxonomic status. Sequences of mitochondrial DNA markers will be used to examine their evolutionary relationships. The expected results would fill the gaps of knowledge in population distribution and infection dynamics of parasitic helminths in the Tianzhu White Yak, which will provide scientific foundation for the effective prevention and control of parasitic helminths of the Tianzhu White Yak.

英文关键词： Tianzhu White Yak;helminth;population distribution;molecular identification;autogenous variation