EB病毒BARTs miRNA在鼻咽癌发生发展中的作用机制研究

项目名称： EB病毒BARTs miRNA在鼻咽癌发生发展中的作用机制研究

项目编号： No.U1405221

项目类型： 联合基金项目

立项/批准年度： 2015

项目学科： 管理科学

项目作者： 潘建基

作者单位： 福建医科大学

项目金额： 256万元

中文摘要： 闽台两地为鼻咽癌高发区，目前尚缺乏稳定高效的肿瘤标志物用于早期诊断及疗效监测。鼻咽癌与EB病毒密切相关，EB病毒表达的产物是关键的肿瘤标志物。我们前期发现：（1）鼻咽癌患者血浆中一些EB病毒BARTs miRNA具有明显的早期诊断和病情监测作用；（2）BARTs miRNA的表达受到病毒和细胞的双重调控；（3）BARTs miRNA具有调控免疫逃逸相关靶基因的功能。在此基础上，本项目进一步进行以下研究：（1）BARTs miRNA 在不同潜伏态、裂解态感染以及鼻咽癌组织中的表达谱分析。（2）探讨BARTs miRNA在鼻咽癌细胞中高表达的调节机制。（3）研究BARTs miRNA 参与鼻咽癌细胞免疫逃逸的机制。（4）阐明BARTs miRNA参与鼻咽癌侵袭转移的作用机制。项目的成果将明确 BARTs miRNA在鼻咽癌发生发展中的作用机制，提供鼻咽癌肿瘤标志物临床转化的理论基础。

中文关键词： 鼻咽癌；EB病毒；microRNA

英文摘要： Fujian and Taiwan are among the high incidence regions for nasopharyngeal carcinoma (NPC). There is no reliable tumor marker for early diagnosis and treatment monitoring of NPC. The hallmark of NPC is the ubiquitously association with Epstein-Barr virus (EBV) and the products of EBV may be potential biomarkers of NPC. Our preliminary studies found that EBV certain species of BARTs miRNA presented in the plasma of NPC patients are closely associated with NPC presence and efficacy of treatment, BARTs miRNA expression is regulated by both viral and cellular mechanisms, and BARTs miRNAs target genes involved in host immune surveillance. Based on these findings, the project will focus on the following aspects: 1) To systematically characterize expression profiles of BARTs miRNA from NPC tissues and EBV-infected cell lines with different latent and lytic viral programs; 2) To delineate molecular mechanism for regulation of BARTs miRNA expression in NPC. 3) To study the roles of BARTs miRNA in immune evasion for NPC. 4) To characterize functions of BARTs miRNA involved in the invasion and metastasis of NPC. The findings from this project will reveal insight for understanding functions of EBV BARTs miRNA in NPC onset and progression, and provide molecular basis for the clinical transformation of these tumor markers.

英文关键词： Nasopharyngeal carcinoma;Epstein-Barr virus ;microRNA