DNA水凝胶-阳离子共轭聚合物杂化荧光纳米粒子的制备及其应用于肿瘤细胞成像与治疗研究

项目名称： DNA水凝胶-阳离子共轭聚合物杂化荧光纳米粒子的制备及其应用于肿瘤细胞成像与治疗研究

项目编号： No.21475031

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 数理科学和化学

项目作者： 成永强

作者单位： 河北大学

项目金额： 85万元

中文摘要： 发展新型多功能杂化荧光纳米粒子（MHFNP）对于肿瘤的早期诊断和治疗等具有重要意义。本项目应用恒温滚环扩增反应产生DNA水凝胶，设计、合成具有不同结构和性能的水溶性阳离子共轭聚合物（CCPs），使DNA水凝胶、CCP与阳离子聚合物聚乙烯亚胺通过静电力作用结合，制备粒径小于100 nm的新型DNA水凝胶-阳离子共轭聚合物MHFNP。系统研究新型MHFNP的生物相容性、功能化修饰、靶向识别活性、细胞成像及细胞杀伤等基础性问题。结合MHFNP中DNA水凝胶优良的柔性骨架结构及生物功能，研究利用MHFNP中DNA的功能化修饰实现细胞的靶向识别。结合MHFNP中CCP高灵敏度荧光成像能力和光动力活性，发展基于新型MHFNP的细胞成像、负载药物治疗和光动力治疗的新技术。项目研究可为肿瘤成像及治疗提供一种新型纳米材料和新的策略，为相关生物医学研究、药物筛选和临床诊断与治疗提供有效的技术平台。

中文关键词： 杂化荧光纳米粒子；DNA水凝胶；阳离子共轭聚合物；细胞成像；肿瘤治疗

英文摘要： It is important to develop novel multifunctional hybrid fluorescnece nanoparticle (MHFNP) for early diagnosis and treatment of tumour. In this project, DNA hydrogel is produced by using isothemal rolling circle ampification. Water cationic conjugated polymers (CCPs) with different structures and properties are designed and synthesized. Subsequently, a new MHFNP based DNA hydrogel and CCP with the size less than 100 nm is prepared by mixing the DNA hydrogel, CCP and polyethyleneimine through electrostatic force interaction. Some fundamental problems about the MHFNP are studied systematically, including biocompatibility, functional modification, target recognition activity, cell imaging and cell damage, etc. By integration of excellent flexible skeleton structure and biological function of DNA hydrogel in MHFNP, cell target recognition is carried out by using the functional modification of DNA in MHFNP. Combining high sensitive fluorescence imaging ability with photodynamic activity of CCP in MHFNP, new techniques based on the MHFNP for cell imaging, loading drugs therapy and photodynamic therapy are developed. This project study can provide a new nanomaterial and new strategy for tumor imaging and therapy. It offers an effective technology platform for the related biomedical research and drug screening as well as the clinical diagnosis and treatment.

英文关键词： hybrid fluorescence nanoparticle;DNA hydrogel;cationic conjugated polymer;cell imaging;tumour therapy