一种对单糖分子识别的新体系研究

项目名称： 一种对单糖分子识别的新体系研究

项目编号： No.20802042

项目类型： 青年科学基金项目

立项/批准年度： 2009

项目学科： 化学工业

项目作者： 冯丽恒

作者单位： 山西大学

项目金额： 18万元

中文摘要： 糖作为生物信息分子，具有重要的生物功能和代谢作用。对糖分子的检测研究既是一个涉及生命科学的理论问题，也是在临床医学上有着应用价值的课题。本项目研究工作旨在得到能够对单糖分子进行有效检测的新型组合体系。研究工作包括两个方面：一是组合体系的构建（有机合成），二是组合体系对单糖的识别（荧光分析）。按照既定目标研究工作得到7个水溶性阴离子聚合物和2个水溶性小分子发光体；得到联吡啶和吡啶两类13个季铵盐阳离子猝灭结合体。在组合体系中，阴离子水溶性化合物作为荧光报告单元，硼酸功能的阳离子季铵盐作为猝灭结合体。二者通过静电作用得到荧光预猝灭体系（荧光"关"）。当单糖进入上述体系中，糖羟基与硼酸基形成可逆性硼酸酯，破坏预猝灭体间的作用，报告单元荧光回复（荧光"开"）。研究工作将聚合物、量子点以及偶氮染料引入，通过对猝灭结合体的类型、功能基数目、位置、空间结构、电荷数等进行修饰得到了高灵敏性的D-葡萄糖检测体系，该体系能很好的应用于糖尿病的检测。另外，得到多个能对D-果糖和阿拉伯识别的组合体，这些组合体可以作为特效探针研究更复杂的生命现象。总之，研究工作从理论上和实际应用上得到了好的结果。

中文关键词： 聚合物；单糖；荧光；识别

英文摘要： As important biological molecule, monosaccharides have important biological functions and metabolism. The research for monosaccharides is important subject for medical diagnostics and basic science fields. The aim of this project is to develop and obtain many effective ensembles for detecting monosaccharides. The research work includes two aspects: one is the construction of combination system (organic synthesis), another is the detection of the systems to monosaccharides (fluorescence analysis). According to the established goals, seven water-soluble anionic polymer and two small molecular compounds are designed and systhesized. Thirteen cationic quaternary ammonium pyridine salts are obtained. Anionic compound is reported group and its fluorescence is modulated by electron transfer from anionic fluorophor to cationic pyridine salt in the system. Cationic pyridine salt acts as both quenchers and receptors. Firstly, the electrostatic interactions between anionic fluorophor and cationic recepor will decrease the fluorescent intensities of anionic fluorophor and form fluorescent "on-off" state of the systems. Next, upon introduction monosaccharide to the "on-off" systems, the effective interaction between cationic receptor and monosaccharide molecule through forming borates can weaken the quenching capability of cationic receptor to anionic fluorophor and result in the fluorescent recoveries of fluorophor (fluorescent "off-on" state of the systems). Through introducting polymer, quantum dots, azo dyes and modifing quencher/receptor, the work obtian highly sensitive ensembles for D-glucose. The D-glucose ensembles can be applied to detect diabetes mellitus. It is feasible to obtain highly selective and sensitive sensing systems for monosaccharides throuth modifing subsituted groups position, space structure, charge number and type of quencher/receptor. In addition, the spectific sensing ensembles for monosaccharide can be used as a probe for studing more complex life phenomenon. In summary, the results of the project is significative on theory research and practical application.

英文关键词： Polymer; Monosaccharide; Fluorescence; Recognition