GSNOR介导蛋白质亚硝基化调控番茄耐碱性机理研究

项目名称： GSNOR介导蛋白质亚硝基化调控番茄耐碱性机理研究

项目编号： No.31501779

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 农业科学

项目作者： 巩彪

作者单位： 山东农业大学

项目金额： 21万元

中文摘要： NO调控植物抗逆性的研究已取得长足进展，但对于NO代谢关键酶GSNOR介导S-nitrosylation调控植物耐碱性的机理知之甚少。本课题组构建了GSNOR不同表达水平的转基因番茄株系，发现GSNOR能够调控内源redox平衡影响植物的S-nitrosylation，结合细胞内redox状态改变和抗性诱导的周期性变化，初步探究了GSNOR控制番茄耐碱性的生理机制。在此基础上，本项目拟通过蛋白质组学深入研究GSNOR介导的S-nitrosylation网络，探求GSNOR调控的关键下游蛋白质和生化途径，通过基因沉默和药物学相结合的研究手段验证这些下游因子在控制番茄耐碱性中的作用。此外，分析GSNOR控制的耐碱关键蛋白的S-nitrosylation程度和作用位点。最终阐明GSNOR介导S-nitrosylation调控番茄耐碱性的机理，为植物抗逆性调控机制的研究奠定理论基础。

中文关键词： 番茄；非生物胁迫；信号转导；调控网络；基因功能

英文摘要： A great progress has been made on the research of NO roles in plants adapting to stress conditions. However, as a key regulator of NO, GSNOR-mediated S-nitrosylation involving in sodic alkali stress tolerance is short of investigation in plant kingdom. We have established transgenic tomato plants with different transcriptional levels of GSNOR, and found that the homeostasis of redox can be regulated by GSNOR that influence S-nitrosylation in plant. Combining with the changes of cellular redox and stress tolerances, the physiological mechanism of GSNOR-mediated sodic alkali stress tolerance in tomato plants was preliminarily studied. Based on this, this project will reveal the GSNOR-regulated S-nitrosylation network by proteomics, in which the downstream proteins and biochemical pathway can be found. Thus, we will use the VIGS technology and exogenous pharmacology method to prove the function of these downstream factors in regulating sodic alkali stress tolerance in tomato plant. In addition, we will study the S-nitrosylation of sodic alkali stress-associated proteins that are regulated by GSNOR. The expected results will make clear the mechanism of sodic alkali stress tolerance regulated by GSNOR-mediated S-nitrosylation, which provides a basis for the improvement of mechanism about stress tolerance in plants.

英文关键词： Tomato;Abiotic stress;Signaling transduction;regulatory network;Gene function