基于生物数据融合的恶性肿瘤中miRNA-ceRNA复合协同调控模式的研究

项目名称： 基于生物数据融合的恶性肿瘤中miRNA-ceRNA复合协同调控模式的研究

项目编号： No.61203264

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 自动化学科

项目作者： 徐娟

作者单位： 哈尔滨医科大学

项目金额： 25万元

中文摘要： 恶性肿瘤的发生发展与miRNA和竞争性内源RNA（ceRNA）密切相关，研究miRNA与ceRNAs在癌症中的复合型协同调控模式是具有挑战意义的课题。本课题从系统生物学角度，通过整合miRNA和靶基因双重表达谱、互作组和功能组等高通量生物数据，借助共调控的功能模块构建了miRNA-ceRNA复合型功能协同调控网络、研究了协同调控作用的潜在机制、疾病miRNA/ceRNA的拓扑特征、从ceRNA角度预测长的非编码RNA和假基因功能的新方法和新技术，旨在实现挖掘癌症发生发展过程中关键的miRNA/ceRNA及miRNA-ceRNA协同调控模块，建立癌症关联的miRNA/ceRNA及协同调控模块识别的生物信息学分析平台，应用方法于乳腺癌、前列腺癌、胶质瘤等癌症数据，挖掘出疾病特异的miRNA和ceRNA及协同调控模块，对推进人类复杂疾病多miRNA多基因研究、探讨癌症发生发展的机理具有重大意义。

中文关键词： 恶性肿瘤；miRNA/lncRNA协同调控网络；ceRNA-ceRNA互作；整合分析；表观调控

英文摘要： It has been known that miRNAs and competitive endogenous RNAs (ceRNAs) are closely associated with oncogenic initiation and progression, so it is a challenge to explore the composite regulation patterns between miRNA and ceRNA.In the subject, we attempt to propose a systematically analyzing strategy to explore the composite regulation patterns between miRNA and ceRNA for malignant tumor, by integrating multidimensional high-throughput biological data, such as paired miRNA-target gene expression profiles, protein interactome and functional information, etc. Here, long non-coding RNAs (lncRNA) and pseudogenes are considered if they are targeted by miRNAs. Firstly, miRNA-ceRNA functional synergistic network is constructed based on co-regulated functional modules, and the specific topological features of known disease miRNAs/ceRNAs are dissected. Then, we systematically analyze the potential mechanisms underlying miRNA/ceRNA synergetic regulations. In addition, the function of lncRNA and pseudogenes is predicted by considering their ceRNA roles.The ultimate goals of our subject are to detect miRNAs/ceRNAs and miRNA-ceRNA functional synergistic modules which play important roles in oncogenesis, and set up a bioinformatic analysis platform. Then, we apply this platform to identify specific cancer-related miRNAs/ceRNAs

英文关键词： malignant tumor；miRNA/lncRNA functional synergistic network；ceRNA-ceRNA interplay；integrative analysis；epigenetic regulation