Maresin1 调控巨噬细胞表型转化在肺损伤中的作用及其机制

项目名称： Maresin1 调控巨噬细胞表型转化在肺损伤中的作用及其机制

项目编号： No.81500064

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 医药、卫生

项目作者： 龚洁

作者单位： 华中科技大学

项目金额： 18万元

中文摘要： 急性肺损伤（ALI）是临床上常见的急危重症的死亡原因，巨噬细胞表型转化在ALI炎症消退过程中发挥着重要的作用。在前期研究发现Maresin 1能减轻小鼠ALI的发生，但其保护机制不清楚。因此，我们进一步提出了“Maresin 1通过增强巨噬细胞表型转化，促进ALI炎症消退”的假说。本研究拟以脂多糖气管内滴注建立小鼠急性肺损伤模型和细胞因子刺激的培养小鼠巨噬细胞RAW264.7为研究对象，动态观察Maresin 1对肺损伤炎症、巨噬细胞吞噬和表型转化功能的影响，同时明确与巨噬细胞表型转化相关的分子事件、发现关键信号通路或调控分子，最终为后续的临床转化提供理论依据。

中文关键词： 肺损伤；Maresin；1；炎症消退；巨噬细胞表型转化

英文摘要： Acute lung injury (ALI) is the major cause of death in acute and severe diseases, and macrophage polarization plays an important role on inflammatory resolution of ALI. Our previous studies have shown that Maresin 1 mitigates lipopolysaccharide-induced ALI in mice, however, the protective mechanism is unclear. Therefore, we established the hypothesis that Maresin 1 promotes resolution of ALI through regulating the macrophage polarization. This study is aimed to use ALI in mice induced by intratracheal instillation of lipopolysaccharide, and inflammatory cytokines-stimulated cultured RAW264.7 as the research objects. We investigate the impacts of Maresin 1 on inflammation of ALI, macrophage phagocytosis and macrophage polarization, and evaluate the cellular and molecular events and vital signaling pathways or regulating signal molecules that contribute to macrophage polarization. This therapy will provide a basis for subsequent clinical translation.

英文关键词： lung injury ;Maresin 1;resolution of inflammation;macrophage polarization