行气活血法中药预知子调节肝癌细胞核糖体蛋白表达的机制探索

项目名称： 行气活血法中药预知子调节肝癌细胞核糖体蛋白表达的机制探索

项目编号： No.81473572

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 医药、卫生

项目作者： 梁超

作者单位： 上海中医药大学

项目金额： 70万元

中文摘要： 项目组在近期的研究中发现行气活血法的常用药- - 预知子（Fruit of Fiverleaf Akebia）提取物会引发肝癌细胞增殖停滞，表达谱芯片检测结果显示大量基因表达发生改变，尤其是部分核糖体蛋白的基因表达变化明显。近期国内外研究表明，核糖体蛋白除了参与蛋白合成之外，还具有多种核糖体外功能，如复制、转录调控和细胞增殖等。据此，中药预知子对肝癌细胞生物学行为的影响可能在一定程度上是通过调节核糖体蛋白基因的表达实现的，综合运用RNAi、过表达、表达谱芯片、MTT、流式细胞仪、RTqPCR、Western bolt等技术，有助于探索待检10个核糖体蛋白在肝癌细胞增殖过程中的作用，及预知子对其调节的意义，这对于深入阐明肝癌恶性增殖的机制和行气活血法代表中药预知子防治肝癌的作用机理，均具有非常重要的学术意义和应用前景。

中文关键词： 原发性肝癌；行气活血法；预知子；核糖体蛋白；表达谱

英文摘要： It has been found in our recent research work that traditional Chinese medicine -Fruit of Fiverleaf Akebia would stop proliferation of liver cancer cells. The results of expression profiling genechip showed that a large number of genes expressions changed, which included some ribosomal proteins (RPs) genes. A lot of recent study revealed that RPs involved in DNA replication, transcriptional regulation and cell proliferation in addition to protein synthesis. Based on the above theories and research results, Fruit of Fiverleaf Akebia may influence primary liver cancer cells' biology function through regulating the expression of RPs. The project would explore the role of 10 RPs in hepatoma cell proliferation process and the regulating effect of Fruit of Fiverleaf Akebia, by means of the methods such as RNAi, over-expression, expression profiling genechip, MTT, flow cytometry (FCM), RTqPCR and Western blot etc. The research work will further elucidate the mechanism of liver cancer and the effect of material medica-Fruit of Fiverleaf Akebia on prevention and treatment of liver cancer. The study also has a very important academic significance and application value.

英文关键词： primary liver cancer;promoting flow of qi and blood circulation;Fruit of Fiverleaf Akebia;ribosomal proteins;Gene expression profiles